Engineering bacterial thiosulfate and tetrathionate sensors for detecting gut inflammation

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dc.citation.articleNumber923en_US
dc.citation.journalTitleMolecular Systems Biologyen_US
dc.citation.volumeNumber13en_US
dc.contributor.authorDaeffler, Kristina N-Men_US
dc.contributor.authorGalley, Jeffrey D.en_US
dc.contributor.authorSheth, Ravi U.en_US
dc.contributor.authorOrtiz-Velez, Laura C.en_US
dc.contributor.authorBibb, Christopher O.en_US
dc.contributor.authorShroyer, Noah F.en_US
dc.contributor.authorBritton, Robert A.en_US
dc.contributor.authorTabor, Jeffrey J.en_US
dc.date.accessioned2017-05-03T19:58:19Zen_US
dc.date.available2017-05-03T19:58:19Zen_US
dc.date.issued2017en_US
dc.description.abstractThere is a groundswell of interest in using genetically engineered sensor bacteria to study gut microbiota pathways, and diagnose or treat associated diseases. Here, we computationally identify the first biological thiosulfate sensor and an improved tetrathionate sensor, both two?component systems from marine Shewanella species, and validate them in laboratory Escherichiaļ¾ coli. Then, we port these sensors into a gut?adapted probiotic E.ļ¾ coli strain, and develop a method based upon oral gavage and flow cytometry of colon and fecal samples to demonstrate that colon inflammation (colitis) activates the thiosulfate sensor in mice harboring native gut microbiota. Our thiosulfate sensor may have applications in bacterial diagnostics or therapeutics. Finally, our approach can be replicated for a wide range of bacterial sensors and should thus enable a new class of minimally invasive studies of gut microbiota pathways.en_US
dc.identifier.citationDaeffler, Kristina N-M, Galley, Jeffrey D., Sheth, Ravi U., et al.. "Engineering bacterial thiosulfate and tetrathionate sensors for detecting gut inflammation." <i>Molecular Systems Biology,</i> 13, (2017) EMBO Press: https://doi.org/10.15252/msb.20167416.en_US
dc.identifier.doihttps://doi.org/10.15252/msb.20167416en_US
dc.identifier.urihttps://hdl.handle.net/1911/94150en_US
dc.language.isoengen_US
dc.publisherEMBO Pressen_US
dc.rightsThis is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.subject.keyworddiagnostic bacteriaen_US
dc.subject.keywordgut inflammationen_US
dc.subject.keywordtetrathionateen_US
dc.subject.keywordthiosulfateen_US
dc.subject.keywordtwo-component systemen_US
dc.titleEngineering bacterial thiosulfate and tetrathionate sensors for detecting gut inflammationen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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