Programming in situ immunofluorescence intensities through interchangeable reactions of dynamic DNA complexes

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dc.citation.firstpage2722
dc.citation.issueNumber18
dc.citation.journalTitleChemBioChem
dc.citation.lastpage2728
dc.citation.volumeNumber13
dc.contributor.authorZimak, Jan
dc.contributor.authorSchweller, Ryan M.
dc.contributor.authorDuose, Dzifa Y.
dc.contributor.authorHittelman, Walter N.
dc.contributor.authorDiehl, Michael R.
dc.date.accessioned2013-08-22T22:52:51Z
dc.date.available2013-08-22T22:52:51Z
dc.date.issued2012
dc.description.abstractThe regulation of antibody reporting intensities is critical to various in situ fluorescence imaging analyses. While such control is often necessary to visualize sparse molecular targets, the ability to tune marker intensities is also essential for highly multiplexed imaging strategies where marker reporting levels must be tuned to both optimize dynamic detection ranges and minimize crosstalk between different signals. Existing chemical amplification approaches generally lack such control. Here, we demonstrate that linear and branched DNA complexes can be designed to function as interchangeable building blocks that can be assembled into organized, fluorescence reporting complexes. We show that the ability to program DNA strand displacement reactions between these complexes offer new opportunities to deterministically tune the number of dyes that are coupled to individual antibodies in order to both increase and controllably balance marker levels within fixed cells.
dc.embargo.termsnone
dc.identifier.citationZimak, Jan, Schweller, Ryan M., Duose, Dzifa Y., et al.. "Programming in situ immunofluorescence intensities through interchangeable reactions of dynamic DNA complexes." <i>ChemBioChem,</i> 13, no. 18 (2012) Wiley-VCH Verlag: 2722-2728. http://dx.doi.org/10.1002/cbic.201200525.
dc.identifier.doihttp://dx.doi.org/10.1002/cbic.201200525
dc.identifier.urihttps://hdl.handle.net/1911/71767
dc.language.isoeng
dc.publisherWiley-VCH Verlag
dc.rightsThis is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Wiley-VCH Verlag.
dc.subject.keywordantibodies
dc.subject.keywordimmunofluorescence
dc.subject.keywordDNA strand displacement
dc.subject.keyworddynamic DNA
dc.subject.keywordmultiplexed detection
dc.titleProgramming in situ immunofluorescence intensities through interchangeable reactions of dynamic DNA complexes
dc.typeJournal article
dc.type.dcmiText
dc.type.publicationpost-print
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