Browsing by Author "Shetler, Samantha"

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    Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
    (JoVE, 2017) Ware, Kathryn E.; Gilja, Shivee; Xu, Shenghan; Shetler, Samantha; Jolly, Mohit K.; Wang, Xueyang; Dewitt, Suzanne Bartholf; Hish, Alexander J.; Jordan, Sarah; Eward, William; Levine, Herbert; Armstrong, Andrew J.; Somarelli, Jason A.; Bioengineering
    We present here a cell culture method for inducing mesenchymal-epithelial transitions (MET) in sarcoma cells based on combined ectopic expression of microRNA-200 family members and grainyhead-like 2 (GRHL2). This method is suitable for better understanding the biological impact of phenotypic plasticity on cancer aggressiveness and treatments.