A synthetic system for asymmetric cell division in Escherichia coli

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dc.contributor.advisorBennett, Matthew R.en_US
dc.contributor.committeeMemberWagner, Daniel S.en_US
dc.creatorMolinari, Saraen_US
dc.date.accessioned2019-07-17T15:59:27Zen_US
dc.date.available2020-02-01T06:01:34Zen_US
dc.date.created2019-08en_US
dc.date.issued2019-07-16en_US
dc.date.submittedAugust 2019en_US
dc.date.updated2019-07-17T15:59:27Zen_US
dc.description.abstractOne defining property of stem cells is their ability to differentiate via asymmetric cell division, in which a stem cell creates a differentiated daughter cell but retains its own phenotype. Here, I describe a synthetic genetic circuit for controlling asymmetric cell division in E. coli in which a progenitor cell creates a differentiated daughter cell while retaining its original phenotype. Specifically, an inducible system was engineered that can bind and segregate plasmid DNA to a single position in the cell. Upon division, the colocalized plasmids are kept by one and only one of the daughter cells. The other daughter cell receives no plasmid DNA and is hence irreversibly differentiated from its sibling. In this way, asymmetric cell division happens though asymmetric plasmid partitioning. This system was further used to achieve physical separation of genetically distinct cells by tying motility to differentiation. Finally, an orthogonal inducible circuit was characterized that enables the simultaneous asymmetric partitioning of two plasmid species, resulting in pluripotent cells that have four distinct differentiated states. These results point the way towards engineering multicellular systems from prokaryotic hosts.en_US
dc.embargo.terms2020-02-01en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationMolinari, Sara. "A synthetic system for asymmetric cell division in Escherichia coli." (2019) Diss., Rice University. <a href="https://hdl.handle.net/1911/106156">https://hdl.handle.net/1911/106156</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/106156en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectsynthetic biologyen_US
dc.subjectE. colien_US
dc.subjectbioengineeringen_US
dc.subjectasymmetric cell divisionen_US
dc.subjectbiologyen_US
dc.subjectbacteriaen_US
dc.subjectengineered bacteriaen_US
dc.titleA synthetic system for asymmetric cell division in Escherichia colien_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentSystems, Synthetic and Physical Biologyen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelDoctoralen_US
thesis.degree.majorSystems/Synthetic/Phys Biologyen_US
thesis.degree.nameDoctor of Philosophyen_US
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