Three-dimensional, "in vitro", cell migration on an artificial substrate: the effects of a collagen coating

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dc.contributor.advisorArmeniades, C. D.en_US
dc.creatorShepherd, James Roberten_US
dc.date.accessioned2018-12-18T21:15:37Zen_US
dc.date.available2018-12-18T21:15:37Zen_US
dc.date.issued1974en_US
dc.description.abstractThis study is part of a research project, which seeks to develop techniques for inducing histogenesis of differentiated tissue on three-dimensional porous substrates. It is concerned with developing a simple, dependable method of coating polyurethane foam with collagen and with defining the characteristics of the collagen coat. The collagen, extracted from bovine tendon, was applied as a one percent dispersion. The collagen coat was directly measured to be 7 to 1A thick by a scanning electron microscope. The coating consisted of intertwining fibrils running in the same general direction. About 8% of the sponge surface area was covered. Adherence tests utilizing the above dispersion, and a glutaraldehyde cross-linked dispersion revealed the collagen coat to be invulnerable to lysis by the tissue or culture medium. The coated sponges exhibited greater invasion by the cells than the uncoated. Elongated cells lying along the coated fibers of the sponge indicated that migration was not random, but rather guided by the collagen, little can be said about improved histological reconstruction of the tissue without a much more extensive sampling. Investigations into optimum sponge pore size are not completed; preliminary results suggest that a pore size of .4 millimeters or larger yields the best growth characteristics.en_US
dc.format.digitalOriginreformatted digitalen_US
dc.format.extent36 ppen_US
dc.identifier.callnoTHESIS CH.E. 1974 SHEPHERDen_US
dc.identifier.citationShepherd, James Robert. "Three-dimensional, "in vitro", cell migration on an artificial substrate: the effects of a collagen coating." (1974) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/103954">https://hdl.handle.net/1911/103954</a>.en_US
dc.identifier.digitalRICE1580en_US
dc.identifier.urihttps://hdl.handle.net/1911/103954en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.titleThree-dimensional, "in vitro", cell migration on an artificial substrate: the effects of a collagen coatingen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentChemical Engineeringen_US
thesis.degree.disciplineEngineeringen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Scienceen_US
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