Polymorphism of microsatellites in coding regions of Dictyostelium discoideum

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dc.contributor.advisorQueller, David C.
dc.contributor.advisorStrassmann, Joan E.
dc.creatorScala, Clea
dc.date.accessioned2009-06-04T06:48:00Z
dc.date.available2009-06-04T06:48:00Z
dc.date.issued2005
dc.description.abstractMicrosatellites are repetitive DNA sequences with high rate of slippage mutations, which cause changes in length. As expected by the neutral theory of molecular evolution the level of polymorphism of these sequences is high when located in non-coding regions that may experience little selection. We tested the hypothesis that triplet repeat microsatellites located in coding regions of the social amoeba Dictyostelium discoideum should have a low level of polymorphism, given the presumably stronger effect on fitness of changes in coding DNA. We analyzed the length of 8 microsatellites located in coding regions of Dictyostellium discoideum in 114 clones of the North America population, divided in 5 subpopulations. Our results showed that each of the eight loci was very highly variable in the population. The lowest range of length variation was 17 repeats (51 bp) for a microsatellite in the ATG1 gene and the maximum range was 78 repeats (234 bp) for a microsatellite in the dimA gene. We tested the possibility that the level of polymorphism was due to population structure. Although present, the population structure was low, and consequently not responsible for the high polymorphism.
dc.format.extent84 p.en_US
dc.format.mimetypeapplication/pdf
dc.identifier.callnoTHESIS ECOL. 2006 SCALA
dc.identifier.citationScala, Clea. "Polymorphism of microsatellites in coding regions of Dictyostelium discoideum." (2005) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/17914">https://hdl.handle.net/1911/17914</a>.
dc.identifier.urihttps://hdl.handle.net/1911/17914
dc.language.isoeng
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.
dc.subjectGenetics
dc.titlePolymorphism of microsatellites in coding regions of Dictyostelium discoideum
dc.typeThesis
dc.type.materialText
thesis.degree.departmentEcology and Evolutionary Biology
thesis.degree.disciplineNatural Sciences
thesis.degree.grantorRice University
thesis.degree.levelMasters
thesis.degree.nameMaster of Arts
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