Single Molecule Studies of Ion-Exchange Chromatography

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dc.contributor.advisorLandes, Christy Fen_US
dc.contributor.committeeMemberWeisman, R. Bruceen_US
dc.contributor.committeeMemberPasquali, Matteoen_US
dc.creatorKisley, Lydiaen_US
dc.date.accessioned2016-01-15T21:28:38Zen_US
dc.date.available2016-01-15T21:28:38Zen_US
dc.date.created2015-05en_US
dc.date.issued2015-04-20en_US
dc.date.submittedMay 2015en_US
dc.date.updated2016-01-15T21:28:38Zen_US
dc.description.abstractAs the pharmeceutical industry moves away from traditional small organic molecules towards biologically-based treatments, ion-exchange separation methods must be investigated to improve the cost and time required for protein purification. Several new single molecule, super-resolution techniques are presented to offer a mechanistic experimental understanding of chromatography unachievable through traditional ensemble-averaged methods. Super-resolution analysis visualizes single protein adsorption kinetics to single, super-resolved ligands, allowing for the first experimental validation of the statistical mechanical stochastic theory of chromatography. Imperative results on the spatial charge-distribution of ligands, reduction of heterogeneity by ionic strength, and tuning of protein/stationary phase interfacial interactions by pH are observed. A common finding that the sterics of the agarose support induces separation heterogeneity leads to super-resolution imaging of the agarose structure and diffusion properties. Finally, the single molecule techniques are applied to several applications beyond protein chromatography to demonstrate the potential for future materials research. Overall, we have shown that single molecule spectroscopy can aid in the mechanistic experimental and theoretical understanding of the ion-exchange chromatographic separation of proteins.en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationKisley, Lydia. "Single Molecule Studies of Ion-Exchange Chromatography." (2015) Diss., Rice University. <a href="https://hdl.handle.net/1911/87860">https://hdl.handle.net/1911/87860</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/87860en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectsuper-resolution spectroscopyen_US
dc.subjectstochastic theoryen_US
dc.subjectbioseparationsen_US
dc.subjectagaroseen_US
dc.titleSingle Molecule Studies of Ion-Exchange Chromatographyen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentChemistryen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelDoctoralen_US
thesis.degree.nameDoctor of Philosophyen_US
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