Construction of recombinant molecules containing RNA processing signals and binding of human snRNPs to some nucleic acids

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dc.contributor.advisorBerget, Susan M.en_US
dc.contributor.committeeMemberPalmer, Grahamen_US
dc.contributor.committeeMemberBennett, Georgeen_US
dc.creatorNees, Daviden_US
dc.date.accessioned2018-12-18T21:24:00Zen_US
dc.date.available2018-12-18T21:24:00Zen_US
dc.date.issued1984en_US
dc.description.abstractThe restriction endonuclease Pst I cleavage site map of Ad2 was generated. Several recombinant molecules were formed, containing Pst I-generated fragments of Ad2 DNA, inserted into the E. coli vector pBR322. These molecules are designed to be useful for the study of RNA processing because they contain signals for splicing and polyadenylation. A filter binding assay was developed to measure the affinity of snRNPs for nucleic acids. The of U1 snRNPs was determined to be on the order of 1 to 11 M for RNA with or without a donor splice junction and for denatured DNA. U2 snRNP also bound RNA containing a donor splice junction with a KD of 1 M. The dissociation of U1 snRNP from denatured DNA was followed and the remainder of the snRNPs dissociated in less than one minute and fifty percent dissociated with a t1/2 of 2 minutes.en_US
dc.format.digitalOriginreformatted digitalen_US
dc.format.extent126 ppen_US
dc.identifier.callnoThesis Biochem. 1984 Neesen_US
dc.identifier.citationNees, David. "Construction of recombinant molecules containing RNA processing signals and binding of human snRNPs to some nucleic acids." (1984) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/104487">https://hdl.handle.net/1911/104487</a>.en_US
dc.identifier.digitalRICE2122en_US
dc.identifier.urihttps://hdl.handle.net/1911/104487en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.titleConstruction of recombinant molecules containing RNA processing signals and binding of human snRNPs to some nucleic acidsen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentBiochemistry and Cell Biologyen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Artsen_US
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