In vivo Fluorescence Imaging with Lensless Microscopes

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dc.contributor.advisorRobinson, Jacob Ten_US
dc.contributor.advisorVeeraraghavan, Ashoken_US
dc.creatorAdams, Jesse Kennethen_US
dc.date.accessioned2019-08-12T14:28:06Zen_US
dc.date.available2020-08-01T05:01:09Zen_US
dc.date.created2019-08en_US
dc.date.issued2019-08-08en_US
dc.date.submittedAugust 2019en_US
dc.date.updated2019-08-12T14:28:06Zen_US
dc.description.abstractFluorescence microscopy is an essential tool for studying the brain. Not only can it provide sub-cellular information about brain structure, but it can also capture dynamic electrical and chemical activity from calcium- and voltage-sensitive indicators. An ideal fluorescence microscope would simultaneously image all the neurons in an animal with the temporal resolution to identify individual action potentials and would not restrict animal behavior. Unfortunately, lenses in traditional microscopes enforce a trade-off between size and weight, resolution, and field-of-view. It is not currently possible to simultaneously achieve cellular resolution, high frame rate, and large fields of view, with a small and lightweight microscope . Recent developments in computational imaging have made it possible to reconstruct images without the use of lenses , thus overcoming many constraints of traditional microscopy. Here we show the first demonstration of a lensless microscope that can perform structural and functional imaging of biological samples in vivo. Specifically, by replacing lenses with an optimized phase mask and computational image reconstruction algorithms we achieve cellular-resolution fluorescence imaging of fixed biological samples . We also demonstrate 2D and 3D in vivo imaging of neurons and muscle cells in millimeter-sized Hydra vulgaris, including measurement of dynamic calcium activity . Finally, we reconstruct stimulus-evoked calcium activity from neurons in mouse cortex . Further miniaturization of this lensless microscope by reducing the size of the electronic packaging will enable flat, and potentially fully implantable devices that can study neural activity over large areas of the brain as animals behave freely. We also anticipate that this new imaging capability can be used in other areas including endoscopy and point-of-care diagnostics, where the small form factor, large field of view, and high temporal resolution will provide advantages compared to current lens-based microscopes.en_US
dc.embargo.terms2020-08-01en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationAdams, Jesse Kenneth. "In vivo Fluorescence Imaging with Lensless Microscopes." (2019) Diss., Rice University. <a href="https://hdl.handle.net/1911/106201">https://hdl.handle.net/1911/106201</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/106201en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectlenslessen_US
dc.subjectmicroscopyen_US
dc.subjectfluorescenceen_US
dc.subjectflaten_US
dc.subjectcalcium imagingen_US
dc.subjectphase masken_US
dc.subjectin vivoen_US
dc.subjectbrainen_US
dc.subjectcompacten_US
dc.subjectlens-freeen_US
dc.subjectmicroscopeen_US
dc.titleIn vivo Fluorescence Imaging with Lensless Microscopesen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentApplied Physicsen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelDoctoralen_US
thesis.degree.majorApplied Physics/Electrical Engen_US
thesis.degree.nameDoctor of Philosophyen_US
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