Metabolism of 5 alpha-cholest-8(14)-en-3 beta-ol-15-one in 15-ketosterol-adapted cells
| dc.creator | Kirkpatrick, Nanda Duhe | en_US |
| dc.date.accessioned | 2007-05-09T18:06:26Z | en_US |
| dc.date.available | 2007-05-09T18:06:26Z | en_US |
| dc.date.issued | 1990 | en_US |
| dc.description.abstract | 5$\alpha$-Cholest-8(14)-en-3$\beta$-ol-15-one is a potent inhibitor of cholesterol biosynthesis in cultured cells. Cells derived from the CHO K-1 cell line have been isolated based on their ability to grow in high concentrations of 5$\alpha$-cholest-8(14)-en-3$\beta$-ol-15-one (up to 40 $\mu$M). These cells, designated as the 15-ketosterol-adapted or K(15) cells, accumulate cytoplasmic lipid-rich regions that appear as inclusions when observed by light microscopy. Results of studies presented herein show that K(15) cells incubated at 40 $\mu$M 5$\alpha$-cholest-8(14)-en-3$\beta$-ol-15-one incorporated almost seven times as much of the 15-ketosterol as K(15) cells incubated at 15 $\mu$M 15-kestosterol. Upon incubation of K(15) cells in lipid-rich medium containing 40 $\mu$M (2,4-$\sp3$H) 5$\alpha$-cholest-8(14)-en-3$\beta$-ol-15-one, $\sim$96% of the radioactivity recovered in an isolated floating lipid layer corresponded to 15-ketosteryl esters. Upon the addition of sodium (1-$\sp{14}$C) oleate (82 $\mu$M) to the incubation medium, the radioactivity recovered in the floating lipid layer corresponded to both 15-ketosteryl esters and triacylglycerols. Following removal of the 15-ketosterol from the growth medium, the K(15) cells excreted 66-88% of the incorporated 15-ketosterol into the medium. | en_US |
| dc.format.mimetype | application/pdf | en_US |
| dc.identifier.callno | Thesis Biochem. 1990 Kirkpatrick | en_US |
| dc.identifier.citation | Kirkpatrick, Nanda Duhe. "Metabolism of 5 alpha-cholest-8(14)-en-3 beta-ol-15-one in 15-ketosterol-adapted cells." (1990) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/13444">https://hdl.handle.net/1911/13444</a>. | en_US |
| dc.identifier.uri | https://hdl.handle.net/1911/13444 | en_US |
| dc.language.iso | eng | en_US |
| dc.rights | Copyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder. | en_US |
| dc.subject | Biochemistry | en_US |
| dc.title | Metabolism of 5 alpha-cholest-8(14)-en-3 beta-ol-15-one in 15-ketosterol-adapted cells | en_US |
| dc.type | Thesis | en_US |
| dc.type.material | Text | en_US |
| thesis.degree.department | Biochemistry and Cell Biology | en_US |
| thesis.degree.discipline | Natural Sciences | en_US |
| thesis.degree.grantor | Rice University | en_US |
| thesis.degree.level | Masters | en_US |
| thesis.degree.name | Master of Arts | en_US |
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