Heterogeneous antibodies against SARS-CoV-2 spike receptor binding domain and nucleocapsid with implications for COVID-19 immunity

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dc.citation.articleNumbere142386en_US
dc.citation.issueNumber18en_US
dc.citation.journalTitleJCI Insighten_US
dc.citation.volumeNumber5en_US
dc.contributor.authorMcAndrews, Kathleen M.en_US
dc.contributor.authorDowlatshahi, Dara P.en_US
dc.contributor.authorDai, Jianlien_US
dc.contributor.authorBecker, Lisa M.en_US
dc.contributor.authorHensel, Janineen_US
dc.contributor.authorSnowden, Laura M.en_US
dc.contributor.authorLeveille, Jennifer M.en_US
dc.contributor.authorBrunner, Michael R.en_US
dc.contributor.authorHolden, Kylie W.en_US
dc.contributor.authorHopkins, Nikolas S.en_US
dc.contributor.authorHarris, Alexandria M.en_US
dc.contributor.authorKumpati, Jerushaen_US
dc.contributor.authorWhitt, Michael A.en_US
dc.contributor.authorLee, J. Jacken_US
dc.contributor.authorOstrosky-Zeichner, Luis L.en_US
dc.contributor.authorPapanna, Rameshaen_US
dc.contributor.authorLeBleu, Valerie S.en_US
dc.contributor.authorAllison, James P.en_US
dc.contributor.authorKalluri, Raghuen_US
dc.date.accessioned2021-08-03T18:33:19Zen_US
dc.date.available2021-08-03T18:33:19Zen_US
dc.date.issued2020en_US
dc.description.abstractEvaluation of potential immunity against the novel severe acute respiratory syndrome (SARS) coronavirus that emerged in 2019 (SARS-CoV-2) is essential for health, as well as social and economic recovery. Generation of antibody response to SARS-CoV-2 (seroconversion) may inform on acquired immunity from prior exposure, and antibodies against the SARS-CoV-2 spike protein receptor binding domain (S-RBD) are speculated to neutralize virus infection. Some serology assays rely solely on SARS-CoV-2 nucleocapsid protein (N-protein) as the antibody detection antigen; however, whether such immune responses correlate with S-RBD response and COVID-19 immunity remains unknown. Here, we generated a quantitative serological ELISA using recombinant S-RBD and N-protein for the detection of circulating antibodies in 138 serial serum samples from 30 reverse transcription PCR–confirmed, SARS-CoV-2–hospitalized patients, as well as 464 healthy and non–COVID-19 serum samples that were collected between June 2017 and June 2020. Quantitative detection of IgG antibodies against the 2 different viral proteins showed a moderate correlation. Antibodies against N-protein were detected at a rate of 3.6% in healthy and non–COVID-19 sera collected during the pandemic in 2020, whereas 1.9% of these sera were positive for S-RBD. Approximately 86% of individuals positive for S-RBD–binding antibodies exhibited neutralizing capacity, but only 74% of N-protein–positive individuals exhibited neutralizing capacity. Collectively, our studies show that detection of N-protein–binding antibodies does not always correlate with presence of S-RBD–neutralizing antibodies and caution against the extensive use of N-protein–based serology testing for determination of potential COVID-19 immunity.en_US
dc.identifier.citationMcAndrews, Kathleen M., Dowlatshahi, Dara P., Dai, Jianli, et al.. "Heterogeneous antibodies against SARS-CoV-2 spike receptor binding domain and nucleocapsid with implications for COVID-19 immunity." <i>JCI Insight,</i> 5, no. 18 (2020) American Society for Clinical Investigation: https://doi.org/10.1172/jci.insight.142386.en_US
dc.identifier.doihttps://doi.org/10.1172/jci.insight.142386en_US
dc.identifier.urihttps://hdl.handle.net/1911/111072en_US
dc.language.isoengen_US
dc.publisherAmerican Society for Clinical Investigationen_US
dc.rightsThis work is licensed under the Creative Commons Attribution 4.0 International License.en_US
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/en_US
dc.titleHeterogeneous antibodies against SARS-CoV-2 spike receptor binding domain and nucleocapsid with implications for COVID-19 immunityen_US
dc.typeJournal articleen_US
dc.type.dcmiTexten_US
dc.type.publicationpublisher versionen_US
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