Anaerobic transformation of 2,4,6-trinitrotoluene by Clostridium acetobutylicum

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dc.contributor.advisorHughes, Joseph B.
dc.creatorKhan, Tariq A.
dc.date.accessioned2009-06-03T23:56:25Z
dc.date.available2009-06-03T23:56:25Z
dc.date.issued1996
dc.description.abstractIn this work, rapid TNT ($>$150 mg/L) transformation by Clostridium acetobutylicum via a reductive pathway to monoaminonitrotoluenes, diaminonitrotoluenes, and on to undetectable end products was observed. The maximum velocities were greatest for TNT reduction, followed by 2A46DNT, 4A26DNT, and 24DA6NT. Initial reduction was preferred at the para position. The measured TNT transformation rates were considerably higher than those observed by others. The transformation of hydroxylamino intermediates was rate limiting. Rates and extents of transformation of TNT and its metabolites were affected by the organism's growth phase. When TNT was added in the early acidogenic phase, rapid transformation to undetectable compounds occurred. When added at the end of exponential growth, TNT and its intermediates were recalcitrant. The decline in transformation was putatively due to ferrodoxin and electron transfer inactivity. The results show potential for this organism to remediate TNT contamination, as in an ex situ bioslurry reactor amended with soluble glucose (sugar).
dc.format.extent120 p.en_US
dc.format.mimetypeapplication/pdf
dc.identifier.callnoTHESIS ENV.SCI. 1996 KHAN
dc.identifier.citationKhan, Tariq A.. "Anaerobic transformation of 2,4,6-trinitrotoluene by Clostridium acetobutylicum." (1996) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/14073">https://hdl.handle.net/1911/14073</a>.
dc.identifier.urihttps://hdl.handle.net/1911/14073
dc.language.isoeng
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.
dc.subjectEnvironmental science
dc.subjectChemical engineering
dc.subjectMicrobiology
dc.subjectBiology
dc.subjectEngineering
dc.titleAnaerobic transformation of 2,4,6-trinitrotoluene by Clostridium acetobutylicum
dc.typeThesis
dc.type.materialText
thesis.degree.departmentEnvironmental Science
thesis.degree.disciplineEngineering
thesis.degree.grantorRice University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science
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