A Novel Human Adipocyte-Derived Basement Membrane for Tissue Engineering Applications

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dc.contributor.advisorNagrath, Deepaken_US
dc.contributor.committeeMemberZygourakis, Kyriacosen_US
dc.contributor.committeeMemberGonzalez, Ramonen_US
dc.contributor.committeeMemberSabek, Omaimaen_US
dc.creatorDamm, Aaronen_US
dc.date.accessioned2012-09-06T00:08:12Zen_US
dc.date.accessioned2012-09-06T00:08:15Zen_US
dc.date.available2012-09-06T00:08:12Zen_US
dc.date.available2012-09-06T00:08:15Zen_US
dc.date.created2012-05en_US
dc.date.issued2012-09-05en_US
dc.date.submittedMay 2012en_US
dc.date.updated2012-09-06T00:08:16Zen_US
dc.description.abstractTissue engineering strategies have traditionally focused on the use of synthetic polymers as support scaffolds for cell growth. Recently, strategies have shifted towards a natural biologically derived scaffold, with the main focus on decellularized organs. Here, we report the development and engineering of a scaffold naturally secreted by human preadipocytes during differentiation. During this differentiation process, the preadipocytes remodel the extracellular matrix by releasing new extracellular proteins. Finally, we investigated the viability of the new basement membrane as a scaffold for tissue engineering using human pancreatic islets, and as a scaffold for soft tissue repair. After identifying the original scaffold material, we sought to improve the yield of material, treating the cell as a bioreactor, through various nutritional and cytokine stimuli. The results suggest that adipocytes can be used as bioreactors to produce a designer-specified engineered human extracellular matrix scaffold for specific tissue engineering applications.en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationDamm, Aaron. "A Novel Human Adipocyte-Derived Basement Membrane for Tissue Engineering Applications." (2012) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/64639">https://hdl.handle.net/1911/64639</a>.en_US
dc.identifier.slug123456789/ETD-2012-05-89en_US
dc.identifier.urihttps://hdl.handle.net/1911/64639en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectAdipocytesen_US
dc.subjectHumalipogelen_US
dc.subjectBioscaffolden_US
dc.subjectExtracellular matrixen_US
dc.subjectGrowth factoren_US
dc.subjectNatural biomaterialsen_US
dc.titleA Novel Human Adipocyte-Derived Basement Membrane for Tissue Engineering Applicationsen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentChemical and Biomolecular Engineeringen_US
thesis.degree.disciplineEngineeringen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Scienceen_US
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