A Novel Human Adipocyte-Derived Basement Membrane for Tissue Engineering Applications

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dc.contributor.advisorNagrath, Deepak
dc.contributor.committeeMemberZygourakis, Kyriacos
dc.contributor.committeeMemberGonzalez, Ramon
dc.contributor.committeeMemberSabek, Omaima
dc.creatorDamm, Aaron
dc.date.accessioned2012-09-06T00:08:12Z
dc.date.accessioned2012-09-06T00:08:15Z
dc.date.available2012-09-06T00:08:12Z
dc.date.available2012-09-06T00:08:15Z
dc.date.created2012-05
dc.date.issued2012-09-05
dc.date.submittedMay 2012
dc.date.updated2012-09-06T00:08:16Z
dc.description.abstractTissue engineering strategies have traditionally focused on the use of synthetic polymers as support scaffolds for cell growth. Recently, strategies have shifted towards a natural biologically derived scaffold, with the main focus on decellularized organs. Here, we report the development and engineering of a scaffold naturally secreted by human preadipocytes during differentiation. During this differentiation process, the preadipocytes remodel the extracellular matrix by releasing new extracellular proteins. Finally, we investigated the viability of the new basement membrane as a scaffold for tissue engineering using human pancreatic islets, and as a scaffold for soft tissue repair. After identifying the original scaffold material, we sought to improve the yield of material, treating the cell as a bioreactor, through various nutritional and cytokine stimuli. The results suggest that adipocytes can be used as bioreactors to produce a designer-specified engineered human extracellular matrix scaffold for specific tissue engineering applications.
dc.format.mimetypeapplication/pdf
dc.identifier.citationDamm, Aaron. "A Novel Human Adipocyte-Derived Basement Membrane for Tissue Engineering Applications." (2012) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/64639">https://hdl.handle.net/1911/64639</a>.
dc.identifier.slug123456789/ETD-2012-05-89
dc.identifier.urihttps://hdl.handle.net/1911/64639
dc.language.isoeng
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.
dc.subjectAdipocytes
dc.subjectHumalipogel
dc.subjectBioscaffold
dc.subjectExtracellular matrix
dc.subjectGrowth factor
dc.subjectNatural biomaterials
dc.titleA Novel Human Adipocyte-Derived Basement Membrane for Tissue Engineering Applications
dc.typeThesis
dc.type.materialText
thesis.degree.departmentChemical and Biomolecular Engineering
thesis.degree.disciplineEngineering
thesis.degree.grantorRice University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science
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