Quantitative FLIM-FRET Measurement of Voltage Dependent Prestin Conformational Changes

Simple item page
dc.contributor.advisorRaphael, Robert M.
dc.contributor.committeeMemberDiehl, Michael R.
dc.contributor.committeeMemberMittleman, Daniel M.
dc.creatorMooney, Chance
dc.date.accessioned2013-09-16T16:01:54Z
dc.date.accessioned2013-09-16T16:01:57Z
dc.date.available2013-09-16T16:01:54Z
dc.date.available2013-09-16T16:01:57Z
dc.date.created2013-05
dc.date.issued2013-09-16
dc.date.submittedMay 2013
dc.date.updated2013-09-16T16:01:57Z
dc.description.abstractThe transmembrane protein prestin forms an integral part of the mammalian sense of hearing by providing the driving force for the electromotility of the outer hair cell, a specialized cell that resides within the cochlea. This provides the cochlea with an ability to amplify mechanical vibrations, allowing for a high degree of sensitivity and selectivity in auditory transduction. The phenomenon, driven by changes in the transmembrane potential, is thought to be the result of conformational changes in self-associating prestin oligomers. We have previously utilized Forster resonance energy transfer (FRET), by both sensitized emission and acceptor photobleach methods, to detect prestin self -association. While these methods can qualitatively confirm prestin-prestin association, determining nanoscale changes in prestin organization requires greater accuracy than either technique provides. In this thesis, a FRET methodology based on fluorescence lifetime imaging (FLIM), detected by time correlated single photon counting (TCSPC), is implemented and utilized to quantitatively measure conformational changes within prestin-prestin oligomers in response to voltage stimulus.
dc.format.mimetypeapplication/pdf
dc.identifier.citationMooney, Chance. "Quantitative FLIM-FRET Measurement of Voltage Dependent Prestin Conformational Changes." (2013) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/72010">https://hdl.handle.net/1911/72010</a>.
dc.identifier.slug123456789/ETD-2013-05-427
dc.identifier.urihttps://hdl.handle.net/1911/72010
dc.language.isoeng
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.
dc.subjectPrestin
dc.subjectFRET
dc.subjectFLIM
dc.subjectPatch-clamp
dc.titleQuantitative FLIM-FRET Measurement of Voltage Dependent Prestin Conformational Changes
dc.typeThesis
dc.type.materialText
thesis.degree.departmentBioengineering
thesis.degree.disciplineEngineering
thesis.degree.grantorRice University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
MOONEY-THESIS.pdf
Size:
1.86 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.61 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Rice University Electronic Theses and Dissertations