Structured Illumination in a Fiber-Optic Microendoscope to Image Nuclear Morphometry in Columnar Epithelium

Simple item page
dc.contributor.advisorRichards-Kortum, Rebecca Raeen_US
dc.contributor.committeeMemberTkaczyk, Tomasz Sen_US
dc.contributor.committeeMemberNatelson, Douglasen_US
dc.contributor.committeeMemberDrezek, Rebekah Aen_US
dc.creatorKeahey, Pelhamen_US
dc.date.accessioned2016-02-04T15:59:22Zen_US
dc.date.available2016-02-04T15:59:22Zen_US
dc.date.created2015-05en_US
dc.date.issued2015-03-23en_US
dc.date.submittedMay 2015en_US
dc.date.updated2016-02-04T15:59:23Zen_US
dc.description.abstractFiber-optic microendoscopes have shown promise to image the changes in nuclear morphometry that accompany the development of precancerous lesions in tissue with squamous epithelium such as in the oral mucosa and cervix. However, fiber-optic microendoscopy image contrast is limited by out-of-focus light generated by scattering within tissue. The scattering coefficient of tissues with columnar epithelium can be greater than that of squamous epithelium resulting in decreased image quality. To address this challenge, I present a small and portable microendoscope system capable of performing optical sectioning using structured illumination (SI) in real-time. Several optical phantoms were developed and used to quantify the sectioning capabilities of the system. Columnar epithelium from cervical tissue specimens was then imaged ex vivo, and I demonstrate that the addition of SI achieves higher image contrast, enabling visualization of nuclear morphology.en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationKeahey, Pelham. "Structured Illumination in a Fiber-Optic Microendoscope to Image Nuclear Morphometry in Columnar Epithelium." (2015) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/88357">https://hdl.handle.net/1911/88357</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/88357en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectMedical optics and biotechnologyen_US
dc.subjectFiber optics imagingen_US
dc.subjectClinical applicationsen_US
dc.subjectEndoscopic imagingen_US
dc.subjectFluorescence microscopyen_US
dc.subjectTissue characterizationen_US
dc.titleStructured Illumination in a Fiber-Optic Microendoscope to Image Nuclear Morphometry in Columnar Epitheliumen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentBioengineeringen_US
thesis.degree.disciplineEngineeringen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.majorApplied Physics/Bioengineeringen_US
thesis.degree.nameMaster of Scienceen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
KEAHEY-DOCUMENT-2015.pdf
Size:
4.04 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 2 of 2
No Thumbnail Available
Name:
PROQUEST_LICENSE.txt
Size:
5.84 KB
Format:
Plain Text
Description:
Download
No Thumbnail Available
Name:
LICENSE.txt
Size:
2.61 KB
Format:
Plain Text
Description:
Download
Collections
Rice University Theses and Dissertations