Amino acid transport in Trypanosome gambiense

Simple item page
dc.contributor.advisorFisher, Frank M.en_US
dc.contributor.advisorRead, Clark P.en_US
dc.creatorHansen, Brian Daviden_US
dc.date.accessioned2018-12-18T21:22:54Zen_US
dc.date.available2018-12-18T21:22:54Zen_US
dc.date.issued1974en_US
dc.description.abstractUsing C-labeled amino acids, absorption of L-lysine, L-arginine, L-glutamate, L-phenylalanine, L-methionine, L-threonine, L-alanine, and L-glycine were studied. All amino acids appeared to be absorbed by both simple diffusion and mediated systems. Inhibitions produced by analogues indicate 5 possible transport loci. Locus A may have 3 Bites, one which transports lysine and the remaining 2 binding glutamate and arginine. When occupied, the latter 2 appear to stimulate the transport of lysine. Methionine and phenylalanine were shown to bind nonproductively at the lysine transport site. Locus B transports threonine, glycine, and alanine with negligible interaction with the other amino acids studied. This locus is partially Na+-sensitive. Locus C transports glutamate primarily. Locus D has 2 transport sites, one for phenylalanine and one for methionine. Threonine shows slight interaction at this site. Locus E also has 2 transport sites, one for lysine and one for arginine. All 8 amino acids were tested for Na+ sensitivity, with only the amino acids entering at Locus B affected. Further evidence for Na+ sensitivity of Locus B was demonstrated in that the uptake of threonine, glycine, and alanine was ouabain-sensitive. The uptake of methionine and phenylalanine was measured in the presence of an amino acid mixture and the observed values agreed with those predicted by calculation according to the technique of Read et al. (1963).en_US
dc.format.digitalOriginreformatted digitalen_US
dc.format.extent83 ppen_US
dc.identifier.callnoThesis Biol. 1974 Hansenen_US
dc.identifier.citationHansen, Brian David. "Amino acid transport in Trypanosome gambiense." (1974) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/104426">https://hdl.handle.net/1911/104426</a>.en_US
dc.identifier.digitalRICE2061en_US
dc.identifier.urihttps://hdl.handle.net/1911/104426en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.titleAmino acid transport in Trypanosome gambienseen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentBiologyen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Artsen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
RICE2061.pdf
Size:
1.65 MB
Format:
Adobe Portable Document Format
Download
Collections
Rice University Theses and Dissertations