Cloning and expressing of rubredoxin oxidoreductase from Clostridium acetobutylicum in Escherichia coli

Simple item page
dc.contributor.advisorRudolph, Frederick B.
dc.creatorGui, Lei
dc.date.accessioned2009-06-04T00:40:38Z
dc.date.available2009-06-04T00:40:38Z
dc.date.issued1995
dc.description.abstractA 550 bp DNA fragment which apparently contains part of the rubredoxin oxidoreductase gene has been amplified by a polymerase chain reaction employing genonic DNA as template. Oligonucleotides used in this amplification were designed based on the multiple alignment of rubredoxin oxidoreductase from related species. An EMBL3 $\lambda$ phage library of C. acetobutylicum genomic DNA was screened by an oligonucleotide hybridization method. The phages which hybridized with the radiolabeled PCR product were subcloned into pUC19 and pACYC184 vectors, with the recombinant plasmids being selected on the basis of white/blue color screening method and the insertional inactivation method, respectively. The probable identity of the pUC19 clone, which was designated RuNC51 and carried a 3.0 kb BglII fragment, was confirmed by rubredoxin oxidoreductase enzyme activity assay. The sequence of RuNC51 showed the same level of similarity to related genes. It also surprisingly showed a 53% similarity to PriA (protein n$\sp\prime$) which is involved in DNA replication.
dc.format.extent63 p.en_US
dc.format.mimetypeapplication/pdf
dc.identifier.callnoTHESIS BIOCHEM. 1995 GUI
dc.identifier.citationGui, Lei. "Cloning and expressing of rubredoxin oxidoreductase from Clostridium acetobutylicum in Escherichia coli." (1995) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/13953">https://hdl.handle.net/1911/13953</a>.
dc.identifier.urihttps://hdl.handle.net/1911/13953
dc.language.isoeng
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.
dc.subjectMolecular biology
dc.titleCloning and expressing of rubredoxin oxidoreductase from Clostridium acetobutylicum in Escherichia coli
dc.typeThesis
dc.type.materialText
thesis.degree.departmentBiochemistry and Cell Biology
thesis.degree.disciplineNatural Sciences
thesis.degree.grantorRice University
thesis.degree.levelMasters
thesis.degree.nameMaster of Arts
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
1377012.PDF
Size:
2.33 MB
Format:
Adobe Portable Document Format
Download
Collections
Rice University Electronic Theses and Dissertations