Cloning and expressing of rubredoxin oxidoreductase from Clostridium acetobutylicum in Escherichia coli

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dc.contributor.advisorRudolph, Frederick B.en_US
dc.creatorGui, Leien_US
dc.date.accessioned2009-06-04T00:40:38Zen_US
dc.date.available2009-06-04T00:40:38Zen_US
dc.date.issued1995en_US
dc.description.abstractA 550 bp DNA fragment which apparently contains part of the rubredoxin oxidoreductase gene has been amplified by a polymerase chain reaction employing genonic DNA as template. Oligonucleotides used in this amplification were designed based on the multiple alignment of rubredoxin oxidoreductase from related species. An EMBL3 $\lambda$ phage library of C. acetobutylicum genomic DNA was screened by an oligonucleotide hybridization method. The phages which hybridized with the radiolabeled PCR product were subcloned into pUC19 and pACYC184 vectors, with the recombinant plasmids being selected on the basis of white/blue color screening method and the insertional inactivation method, respectively. The probable identity of the pUC19 clone, which was designated RuNC51 and carried a 3.0 kb BglII fragment, was confirmed by rubredoxin oxidoreductase enzyme activity assay. The sequence of RuNC51 showed the same level of similarity to related genes. It also surprisingly showed a 53% similarity to PriA (protein n$\sp\prime$) which is involved in DNA replication.en_US
dc.format.extent63 p.en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.callnoTHESIS BIOCHEM. 1995 GUIen_US
dc.identifier.citationGui, Lei. "Cloning and expressing of rubredoxin oxidoreductase from Clostridium acetobutylicum in Escherichia coli." (1995) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/13953">https://hdl.handle.net/1911/13953</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/13953en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectMolecular biologyen_US
dc.titleCloning and expressing of rubredoxin oxidoreductase from Clostridium acetobutylicum in Escherichia colien_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentBiochemistry and Cell Biologyen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Artsen_US
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