Optimization of Fast Multiplexed Magnetogenetics

dc.contributor.advisorRobinson, Jacoben_US
dc.creatorSebesta, Charlesen_US
dc.date.accessioned2021-05-03T20:58:00Zen_US
dc.date.available2021-11-01T05:01:12Zen_US
dc.date.created2021-05en_US
dc.date.issued2021-04-30en_US
dc.date.submittedMay 2021en_US
dc.date.updated2021-05-03T20:58:00Zen_US
dc.description.abstractPrecisely timed activation of genetically targeted cells is a powerful tool for studying neural circuits and controlling cell-based therapies. Magnetic control of cell activity or “magnetogenetics” using magnetic nanoparticle heating of temperature-sensitive ion channels enables remote, non-invasive activation of neurons for deep-tissue applications and studies of freely behaving animals. However, the in vivo response time of thermal magnetogenetics is currently tens of seconds, which prevents the precise temporal modulation of neural activity similar to light-based optogenetics. Moreover, magnetogenetics has not provided a means to selectively activate multiple channels to drive behavior. Here I demonstrate that by combining magnetic nanoparticles with a rate-sensitive thermoreceptor (TRPA1-A) it is possible to achieve sub-second behavioral responses in Drosophila melanogaster. Additionally, I have identified novel TRPA1 channels that show promise of translating this work into mammalian systems. Furthermore, by tuning the properties of magnetic nanoparticles to respond to different magnetic field strengths and frequencies, I can achieve fast, multi-channel stimulation, analogous to optogenetic stimulation with different wavelengths of light. These results bring magnetogenetics closer to the temporal resolution and multiplexed stimulation possible with optogenetics while maintaining the minimal invasiveness and deep-tissue stimulation only possible by magnetic control.en_US
dc.embargo.terms2021-11-01en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationSebesta, Charles. "Optimization of Fast Multiplexed Magnetogenetics." (2021) Diss., Rice University. <a href="https://hdl.handle.net/1911/110398">https://hdl.handle.net/1911/110398</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/110398en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectMagnetogeneticsen_US
dc.subjectAlternating Magnetic fielden_US
dc.subjectNanoparticlesen_US
dc.subjectNeuroengineeringen_US
dc.subjectMolecular Biologyen_US
dc.subjectDrosophilaen_US
dc.subjectTRPA1en_US
dc.subjectThermally sensitive ion channelsen_US
dc.subjectNeuroscienceen_US
dc.titleOptimization of Fast Multiplexed Magnetogeneticsen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentBioengineeringen_US
thesis.degree.disciplineEngineeringen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelDoctoralen_US
thesis.degree.nameDoctor of Philosophyen_US
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