Oxygen transport by hemoglobin-based blood substitutes

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dc.contributor.advisorHellums, J. Daviden_US
dc.creatorPage, Thomas Careyen_US
dc.date.accessioned2009-06-04T08:46:33Zen_US
dc.date.available2009-06-04T08:46:33Zen_US
dc.date.issued1997en_US
dc.description.abstractA dual track approach has been applied to the study of oxygen transport behavior of erythrocyte/hemoglobin mixtures flowing in microvessels. The method includes experimental and theoretical modeling of the microcirculation. An experimental in vitro 27 $\mu$m diameter capillary model was developed to provide detailed oxygen flux measurements for homeglobin solutions, erythrocyte suspensions, and erythrocyte/hemoglobin solution mixtures. The experimental apparatus includes computerized data acquisiton and control coupled to a Leitz Toolmaker Microscope-based, dual wavelength microspectrophotomer. Fractional oxygen saturation may be determined for various axial positions, and the resulting experimental data have been shown to agree well with simulations calculated from previously developed theoretical models of oxygen transport in hemoglobin solutions and rbc suspensions. Direct comparison of hemoglobin solutions with rbc suspensions of the same overall hemoglobin concentration and oxygen affinity shows that hemoglobin solutions are more efficient transporters of oxygen. Experiments on polymerized hemoglobin suggests that some heme pockets may have not be available for oxygen transport. The quality and quantity of the experimental data represent a significant improvement over previous experimental designs, and the results confirm existing oxygen transport models. Ultrapurified, native and polymerized bovine hemoglobin/red blood cell mixtures were studied in the capillary. Dose response plots were generated by varying the extracellular to intracellular hemoglobin distribution ratio. Increased extracellular hemoglobin concentration increased oxygen transport efficiency for both uptake and release. When only 10% of the total hemoglobin was extracellular, half of the increased efficiency of pure hemoglobin solutions was reached. When half the hemoglobin was extracellular, the mixtures behaved like hemoglobin solutions. A mathematical model of the mixture experiments initially failed to fully predict the enhancement of oxygen uptake, but well described release experiments. Consideration of shear induced augmentation led to a new hypothesis for the relative importance of extracellular mixing in rbc/hemoglobin mixtures flowing in arteriolar sized conduits. The resulting semi-predictive model matches experimental data for a variety of conditions. This model may be used to predict performance of hemoglobin-based blood substitutes.en_US
dc.format.extent199 p.en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.callnoTHESIS CH.E. 1997 PAGEen_US
dc.identifier.citationPage, Thomas Carey. "Oxygen transport by hemoglobin-based blood substitutes." (1997) Diss., Rice University. <a href="https://hdl.handle.net/1911/19193">https://hdl.handle.net/1911/19193</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/19193en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectBiomedical engineeringen_US
dc.subjectChemical engineeringen_US
dc.titleOxygen transport by hemoglobin-based blood substitutesen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentChemical Engineeringen_US
thesis.degree.disciplineEngineeringen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelDoctoralen_US
thesis.degree.nameDoctor of Philosophyen_US
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