Engineering the Production of Itaconic Acid in Escherichia coli

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dc.contributor.advisorBennett, George N.
dc.creatorLin, Tao
dc.date.accessioned2013-03-08T00:36:00Z
dc.date.available2013-03-08T00:36:00Z
dc.date.issued2011
dc.description.abstractItaconic acid (IA) has many versatile applications in science and medicine. Engineering Escherichia coli can provide a reliable route for IA production, where cis -aconitate decarboxylase (CAD) is one essential enzyme in the process. The synthetic cad gene has been cloned into various vectors to improve the expression rate and folding activities. The strain bearing plasmid pTrc99a- cad displays a significant amount of IA production, while the CAD protein expressed from the same plasmid displays the highest enzymatic activity in vitro. To improve the reliability of forming the cis -aconitate intermediate, the aconitase genes from Aspergillus terreus have been cloned into the pTrc99a vector. The expression of aconitate hydratase leads to a peak at 10.3 min in the HPLC spectra after 4 hours incubation. The peak will be analyzed to determine whether it is cis -aconitate. The obtained data will provide data on possible metabolic pathways of IA production in Aspergillus terreus.
dc.format.extent75 p.en_US
dc.format.mimetypeapplication/pdf
dc.identifier.callnoTHESIS BIOCHEM. 2011 LIN
dc.identifier.citationLin, Tao. "Engineering the Production of Itaconic Acid in Escherichia coli." (2011) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/70322">https://hdl.handle.net/1911/70322</a>.
dc.identifier.digitalLinTen_US
dc.identifier.urihttps://hdl.handle.net/1911/70322
dc.language.isoeng
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.
dc.subjectApplied sciences
dc.subjectPure sciences
dc.subjectBiochemistry
dc.subjectChemical engineering
dc.titleEngineering the Production of Itaconic Acid in Escherichia coli
dc.typeThesis
dc.type.materialText
thesis.degree.departmentBiochemistry and Cell Biology
thesis.degree.disciplineNatural Sciences
thesis.degree.grantorRice University
thesis.degree.levelMasters
thesis.degree.nameMaster of Arts
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