Single Molecule Studies of Dynamics at Polymeric Film Interfaces

Simple item page
dc.contributor.advisorLandes, Christy F
dc.creatorTauzin, Lawrence J
dc.date.accessioned2017-08-03T17:36:11Z
dc.date.available2017-08-03T17:36:11Z
dc.date.created2016-05
dc.date.issued2016-04-21
dc.date.submittedMay 2016
dc.date.updated2017-08-03T17:36:11Z
dc.description.abstractFiltration and separations play large roles in many industries yet no complete mechanistic interpretation of these phenomena exists. The development of experimental methods capable of characterizing dynamics at the single molecule level has opened the door to reexamining these heavily studied processes. By overcoming ensemble averaging mechanistic understandings that account for heterogeneities in these systems can be formulated. In this work, single molecule spectroscopic methods along with advanced particle tracking and localization algorithms are used to directly observe the interaction of single molecule probes with polymer films. The electrostatic contribution to interfacial interactions was evaluated by using the post-assembly tunability of polyelectrolyte multilayers and probes of varying charge. Multimodal transport mechanisms of protein interactions with functionalized nylon membranes were also discovered. Finally, the groundwork for a kinetic model of protein adsorption to a thin film was developed. A major conclusion was that even in ion-exchange systems in which electrostatic interactions are considered the most important variable, complex interactions between the adsorbents and the substrate are not insignificant in polymer membrane based systems. Additionally, interfacial transport in nearly all systems is characterized, not by 2 dimensional surface diffusion or activated hop diffusion, but by desorption mediated diffusion involving repeat adsorption events punctuated by periods of immobilization, the extent of which can be controlled using solution conditions.
dc.format.mimetypeapplication/pdf
dc.identifier.citationTauzin, Lawrence J. "Single Molecule Studies of Dynamics at Polymeric Film Interfaces." (2016) Diss., Rice University. <a href="https://hdl.handle.net/1911/96567">https://hdl.handle.net/1911/96567</a>.
dc.identifier.urihttps://hdl.handle.net/1911/96567
dc.language.isoeng
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.
dc.subjectSingle Molecule
dc.subjectInterfacial Transport
dc.subjectInterfacial Dynamics
dc.subjectPolymer Films
dc.subjectSingle Molecule Microscopy
dc.subjectFluorescence
dc.subjectSeparations
dc.titleSingle Molecule Studies of Dynamics at Polymeric Film Interfaces
dc.typeThesis
dc.type.materialText
thesis.degree.departmentChemistry
thesis.degree.disciplineNatural Sciences
thesis.degree.grantorRice University
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
TAUZIN-DOCUMENT-2016.pdf
Size:
3.36 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 2 of 2
No Thumbnail Available
Name:
PROQUEST_LICENSE.txt
Size:
5.84 KB
Format:
Plain Text
Description:
Download
No Thumbnail Available
Name:
LICENSE.txt
Size:
2.61 KB
Format:
Plain Text
Description:
Download
Collections
Rice University Electronic Theses and Dissertations