Development and Characterization of Split Methyl Halide Transferase for Biosensor Applications in Soil

Simple item page
dc.contributor.advisorSilberg, Jonathan J.en_US
dc.contributor.advisorBennett, George N.en_US
dc.creatorHuh, Dongkuken_US
dc.date.accessioned2019-09-12T21:21:15Zen_US
dc.date.available2019-09-12T21:21:15Zen_US
dc.date.created2019-08en_US
dc.date.issued2019-09-10en_US
dc.date.submittedAugust 2019en_US
dc.date.updated2019-09-12T21:21:15Zen_US
dc.description.abstractSoil microorganisms contribute to agricultural productivity by forming symbiosis with plant roots and affecting the solubility of soil nutrients. Microbiological activity in soil can be monitored directly by coupling the expression of a methyl halide transferase (MHT) to a conditional reporter. This unique bioreporter can provide information about microbial sensing and behaviors without disrupting soil. However, MHT reporting has only been used to report on slow transcriptional processes, limiting its utility. To overcome this challenge, I have created a split MHT (SMHT) that consists of two MHT fragments that are only functional when reconstituted. This SMHT was rationally designed using family sequence information and shown to exhibit MHT-fragment complementation upon fusion to different pairs of interacting proteins. Furthermore, a rapamycin biosensor was constructed that links the detection of rapamycin, a secondary metabolite synthesized by a soil microorganism, to SMHT fragment complementation. The SMHT should be generally useful for reporting on conditional protein-protein interactions in hard-to-image soils and sediments.en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationHuh, Dongkuk. "Development and Characterization of Split Methyl Halide Transferase for Biosensor Applications in Soil." (2019) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/107401">https://hdl.handle.net/1911/107401</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/107401en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectMethyl halide transferaseen_US
dc.subjectMultiple sequence alignmenten_US
dc.subjectProtein fragment complementation assayen_US
dc.subjectGas bioreporteren_US
dc.subjectProtein engineeringen_US
dc.titleDevelopment and Characterization of Split Methyl Halide Transferase for Biosensor Applications in Soilen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentBiochemistry and Cell Biologyen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.majorProtein engineering and Synthetic Biologyen_US
thesis.degree.nameMaster of Scienceen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
HUH-DOCUMENT-2019.pdf
Size:
1.12 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 2 of 2
No Thumbnail Available
Name:
PROQUEST_LICENSE.txt
Size:
5.83 KB
Format:
Plain Text
Description:
Download
No Thumbnail Available
Name:
LICENSE.txt
Size:
2.6 KB
Format:
Plain Text
Description:
Download
Collections
Rice University Theses and Dissertations