Depth-sensitive optical spectroscopy for noninvasive diagnosis of oral neoplasia

Simple item page
dc.contributor.advisorRichards-Kortum, Rebecca Raeen_US
dc.creatorSchwarz, Richard Alanen_US
dc.date.accessioned2011-07-25T02:05:50Zen_US
dc.date.available2011-07-25T02:05:50Zen_US
dc.date.issued2010en_US
dc.description.abstractOral cancer is the 11th most common cancer in the world. Cancers of the oral cavity and oropharynx account for more than 7,500 deaths each year in the United States alone. Major advances have been made in the management of oral cancer through the combined use of surgery, radiotherapy and chemotherapy, improving the quality of life for many patients; however, these advances have not led to a significant increase in survival rates, primarily because diagnosis often occurs at a late stage when treatment is more difficult and less successful. Accurate, objective, noninvasive methods for early diagnosis of oral neoplasia are needed. Here a method is presented to noninvasively evaluate oral lesions using depth-sensitive optical spectroscopy (DSOS). A ball lens coupled fiber-optic probe was developed to enable preferential targeting of different depth regions in the oral mucosa. Clinical studies of the diagnostic performance of DSOS in 157 subjects were carried out in collaboration with the University of Texas M. D. Anderson Cancer Center. An overall sensitivity of 90% and specificity of 89% were obtained for nonkeratinized oral tissue relative to histopathology. Based on these results a compact, portable version of the clinical DSOS device with real-time automated diagnostic capability was developed. The portable device was tested in 47 subjects and a sensitivity of 82% and specificity of 83% were obtained for nonkeratinized oral tissue. The diagnostic potential of multimodal platforms incorporating DSOS was explored through two pilot studies. A pilot study of DSOS in combination with widefield imaging was carried out in 29 oral cancer patients, resulting in a combined sensitivity of 94% and specificity of 69%. Widefield imaging and spectroscopy performed slightly better in combination than each method performed independently. A pilot study of DSOS in combination with the optical contrast agents 2-NBDG, EGF-Alexa 647, and proflavine was carried out in resected tissue specimens from 15 oral cancer patients. Improved contrast between neoplastic and healthy tissue was observed using 2-NBDG and EGF-Alexa 647.en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.callnoTHESIS BIOENG. 2010 SCHWARZen_US
dc.identifier.citationSchwarz, Richard Alan. "Depth-sensitive optical spectroscopy for noninvasive diagnosis of oral neoplasia." (2010) Diss., Rice University. <a href="https://hdl.handle.net/1911/62061">https://hdl.handle.net/1911/62061</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/62061en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectBiomedical engineeringen_US
dc.subjectOpticsen_US
dc.subjectBiophysicsen_US
dc.titleDepth-sensitive optical spectroscopy for noninvasive diagnosis of oral neoplasiaen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentBioengineeringen_US
thesis.degree.disciplineEngineeringen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelDoctoralen_US
thesis.degree.nameDoctor of Philosophyen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
3421192.PDF
Size:
7.87 MB
Format:
Adobe Portable Document Format
Download
Collections
Rice University Theses and Dissertations