Targeted Transcriptional Activation Using a CRISPR-Associated Transposon System

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dc.contributor.advisorChappell, James E
dc.contributor.advisorLoveless, Theresa B
dc.creatorGarza Elizondo, Andrea M
dc.date.accessioned2024-05-21T20:44:13Z
dc.date.available2024-05-21T20:44:13Z
dc.date.created2024-05
dc.date.issued2024-01-25
dc.date.submittedMay 2024
dc.date.updated2024-05-21T20:44:13Z
dc.description.abstractSynthetic perturbation of gene expression is central to our ability to reliably uncover genotype-phenotype relationships in microbes. Here, I present a novel gene transcription activation strategy that uses the Vibrio cholerae CRISPR-Associated Transposon (CAST) system to selectively insert promoter elements upstream of genes of interest. Through this strategy, I show robust activation of both recombinant and endogenous genes across the Escherichia coli chromosome. I then demonstrate precise tuning of expression levels by exchanging the promoter elements being inserted. Finally, I demonstrate that CAST activation can be used to synthetically induce ampicillin-resistant phenotypes in E. coli.
dc.format.mimetypeapplication/pdf
dc.identifier.citationElizondo, Andrea Garza. Targeted Transcriptional Activation Using a CRISPR-Associated Transposon System. (2024). PhD diss., Rice University. https://hdl.handle.net/1911/116076
dc.identifier.urihttps://hdl.handle.net/1911/116076
dc.language.isoeng
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.
dc.subjectCRISPR-Associated Transposon
dc.subjectCRISPR
dc.subjectTranscriptional Activation
dc.titleTargeted Transcriptional Activation Using a CRISPR-Associated Transposon System
dc.typeThesis
dc.type.materialText
thesis.degree.departmentBiochemistry and Cell Biology
thesis.degree.disciplineNatural Sciences
thesis.degree.grantorRice University
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy
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