NOA1: A Tool for Understanding Nitric Oxide Accumulation and Fosmidomycin Resistance in Arabidopsis

Simple item page
dc.contributor.advisorBraam, Janeten_US
dc.creatorVan Ree, Kalieen_US
dc.date.accessioned2013-03-08T00:39:47Zen_US
dc.date.available2013-03-08T00:39:47Zen_US
dc.date.issued2012en_US
dc.description.abstractChloroplasts generate important cellular signals and synthesize diverse products. The chloroplast-localized protein, Nitric Oxide Associated-1 (NOA1), is implicated in nitric oxide (NO) accumulation and linked to the methylerythritol phosphate (MEP) pathway, but its role is undefined. I report that NOA1 is not essential for NO accumulation because the noa1 mutant accumulates NO when provided sucrose-supplemented media. Therefore, chloroplast function and fixed carbon, but not NOA1 are likely critical for plant NO accumulation. noa1 is also resistant to fosmidomycin, an inhibitor of the MEP pathway. This phenotype led to uncovering a potential link between the MEP and tetrapyrrole pathways. I report that fosmidomycin toxicity is light dependent and reduced by phytol supplementation. Downregulation of the tetrapyrrole pathway enhances fosmidomycin resistance, suggesting that reduced tetrapyrrole biosynthesis alleviates fosmidomycin toxicity. These findings reveal new insight into how impairment of the MEP pathway affects plants and the importance of metabolic balance for chloroplast function.en_US
dc.format.extent67 p.en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.callnoTHESIS BIOCHEM. 2012 VAN REEen_US
dc.identifier.citationVan Ree, Kalie. "NOA1: A Tool for Understanding Nitric Oxide Accumulation and Fosmidomycin Resistance in Arabidopsis." (2012) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/70480">https://hdl.handle.net/1911/70480</a>.en_US
dc.identifier.digitalVanReeKen_US
dc.identifier.urihttps://hdl.handle.net/1911/70480en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectBiological sciencesen_US
dc.subjectPlant biologyen_US
dc.titleNOA1: A Tool for Understanding Nitric Oxide Accumulation and Fosmidomycin Resistance in Arabidopsisen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentBiochemistry and Cell Biologyen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Artsen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
VanReeK.pdf
Size:
3.83 MB
Format:
Adobe Portable Document Format
Download
Collections
Rice University Theses and Dissertations