NOA1: A Tool for Understanding Nitric Oxide Accumulation and Fosmidomycin Resistance in Arabidopsis

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dc.contributor.advisorBraam, Janet
dc.creatorVan Ree, Kalie
dc.date.accessioned2013-03-08T00:39:47Z
dc.date.available2013-03-08T00:39:47Z
dc.date.issued2012
dc.description.abstractChloroplasts generate important cellular signals and synthesize diverse products. The chloroplast-localized protein, Nitric Oxide Associated-1 (NOA1), is implicated in nitric oxide (NO) accumulation and linked to the methylerythritol phosphate (MEP) pathway, but its role is undefined. I report that NOA1 is not essential for NO accumulation because the noa1 mutant accumulates NO when provided sucrose-supplemented media. Therefore, chloroplast function and fixed carbon, but not NOA1 are likely critical for plant NO accumulation. noa1 is also resistant to fosmidomycin, an inhibitor of the MEP pathway. This phenotype led to uncovering a potential link between the MEP and tetrapyrrole pathways. I report that fosmidomycin toxicity is light dependent and reduced by phytol supplementation. Downregulation of the tetrapyrrole pathway enhances fosmidomycin resistance, suggesting that reduced tetrapyrrole biosynthesis alleviates fosmidomycin toxicity. These findings reveal new insight into how impairment of the MEP pathway affects plants and the importance of metabolic balance for chloroplast function.
dc.format.extent67 p.en_US
dc.format.mimetypeapplication/pdf
dc.identifier.callnoTHESIS BIOCHEM. 2012 VAN REE
dc.identifier.citationVan Ree, Kalie. "NOA1: A Tool for Understanding Nitric Oxide Accumulation and Fosmidomycin Resistance in Arabidopsis." (2012) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/70480">https://hdl.handle.net/1911/70480</a>.
dc.identifier.digitalVanReeKen_US
dc.identifier.urihttps://hdl.handle.net/1911/70480
dc.language.isoeng
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.
dc.subjectBiological sciences
dc.subjectPlant biology
dc.titleNOA1: A Tool for Understanding Nitric Oxide Accumulation and Fosmidomycin Resistance in Arabidopsis
dc.typeThesis
dc.type.materialText
thesis.degree.departmentBiochemistry and Cell Biology
thesis.degree.disciplineNatural Sciences
thesis.degree.grantorRice University
thesis.degree.levelMasters
thesis.degree.nameMaster of Arts
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