Elucidating the Roles of PEX19 and Prenylation in Arabidopsis Peroxisomes

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dc.contributor.advisorBartel, Bonnieen_US
dc.contributor.committeeMemberShamoo, Yousifen_US
dc.contributor.committeeMemberFarach-Carson, Cindyen_US
dc.contributor.committeeMemberBraam, Janeten_US
dc.contributor.committeeMemberRudgers, Jennifer A.en_US
dc.creatorStoddard, Jerraden_US
dc.date.accessioned2012-09-05T23:55:39Zen_US
dc.date.accessioned2012-09-05T23:56:36Zen_US
dc.date.available2013-09-05T05:10:05Zen_US
dc.date.created2012-05en_US
dc.date.issued2012-09-05en_US
dc.date.submittedMay 2012en_US
dc.date.updated2012-09-05T23:56:36Zen_US
dc.description.abstractPeroxisomes are organelles originating from the endoplasmic reticulum. Peroxisome biogenesis requires multiple peroxins, including PEX19, a prenylated protein that helps deliver peroxisomal membrane proteins in yeast and mammals. Arabidopsis thaliana PEX19 is encoded by two isogenes, PEX19A and PEX19B. I demonstrate that pex19A and pex19B insertional mutants lack obvious abberant physiological phenotypes. I provide evidence that pex19A pex19B double mutants are inviable, that PEX19B is more abundant than PEX19A in young seedlings, that Arabidopsis PEX19 is farnesylated in vivo, and that YFP-PEX19 predominantly associates with what appears to be a subcellular membrane regardless of its prenylation state. I show that farnesyltransferase mutants apparently contain only non-prenylated PEX19 and lack phenotypes that would indicate inefficient peroxisome activity. My analysis of PEX19 suggests that PEX19 prenylation is dispensable for peroxisome biogenesis, and has generated tools for future studies of the earliest steps in peroxisome biogenesis in plants.en_US
dc.embargo.terms2013-09-05T05:00:00Zen_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationStoddard, Jerrad. "Elucidating the Roles of PEX19 and Prenylation in Arabidopsis Peroxisomes." (2012) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/64615">https://hdl.handle.net/1911/64615</a>.en_US
dc.identifier.slug123456789/ETD-2012-05-58en_US
dc.identifier.urihttps://hdl.handle.net/1911/64615en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectAuxinen_US
dc.subjectPeroxinen_US
dc.subjectPeroxisomesen_US
dc.subjectArabidopsisen_US
dc.titleElucidating the Roles of PEX19 and Prenylation in Arabidopsis Peroxisomesen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentBiochemistry and Cell Biologyen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Artsen_US
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