Design and Benchmarking of CRISPR/Cas Tools for Transcriptional Regulation in Human Cells

Simple item page
dc.contributor.advisorHilton, Isaac Ben_US
dc.contributor.advisorBennett, Matthewen_US
dc.creatorEscobar Galdamez, Mario Den_US
dc.date.accessioned2023-08-09T18:31:15Zen_US
dc.date.created2023-05en_US
dc.date.issued2023-04-20en_US
dc.date.submittedMay 2023en_US
dc.date.updated2023-08-09T18:31:15Zen_US
dc.description.abstractCRISPR/Cas systems have revolutionized biomedical research by providing easily programmable nucleic acid-targeting proteins. However, the discovery of different CRISPR systems in various organisms and their rapid proliferation in research settings have crowded the field and ultimately complicated the use of CRISPR tools. During my research, I investigated how different CRISPR/Cas9 design strategies impact the efficiency of these tools. Additionally, I quantified and compared the efficacy of CRISPR/Cas systems from a variety of different species for genome editing and transcriptional control. Finally, I leveraged these optimized design parameters to create a new synthetic system for controlling the generation of new mitochondria within living human cells.en_US
dc.embargo.lift2024-05-01en_US
dc.embargo.terms2024-05-01en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationEscobar Galdamez, Mario D. "Design and Benchmarking of CRISPR/Cas Tools for Transcriptional Regulation in Human Cells." (2023) Diss., Rice University. <a href="https://hdl.handle.net/1911/115139">https://hdl.handle.net/1911/115139</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/115139en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectCRISPRen_US
dc.subjectCRISPRaen_US
dc.subjectEpigeneticsen_US
dc.titleDesign and Benchmarking of CRISPR/Cas Tools for Transcriptional Regulation in Human Cellsen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentBiochemistry and Cell Biologyen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelDoctoralen_US
thesis.degree.nameDoctor of Philosophyen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ESCOBARGALDAMEZ-DOCUMENT-2023.pdf
Size:
11.03 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 2 of 2
No Thumbnail Available
Name:
PROQUEST_LICENSE.txt
Size:
5.85 KB
Format:
Plain Text
Description:
Download
No Thumbnail Available
Name:
LICENSE.txt
Size:
2.62 KB
Format:
Plain Text
Description:
Download
Collections
Rice University Theses and Dissertations