Engineering Escherichia coli for the production of polyketide-based platform chemicals

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dc.contributor.advisorGonzalez, Ramonen_US
dc.creatorPark, Johnen_US
dc.date.accessioned2013-03-08T00:37:28Zen_US
dc.date.available2013-03-08T00:37:28Zen_US
dc.date.issued2012en_US
dc.description.abstractThe current chemical industry produces a diverse array of industrial chemicals from a handful of highly reduced byproducts (termed "platform chemicals") derived from oil refining. However, petroleum is a non-renewable resource, and increases in its cost have created pressure to convert the chemical industry into one that is renewable to ensure its long-term viability. To complete this objective, one approach is the conversion of biomass to platform chemicals through fermentation by Escherichia coli . One such platform chemical is methyl ketone, which can be readily converted to dienes that can directly replace existing platform chemicals such as ethylene. To bestow non-native methyl ketone production capability to E. coli from glucose, the polyketide biosynthesis pathway was exploited in conjunction with grafting in a heterologous methyl ketone synthesis pathway found in wild tomato species Solanum habrochaites to produce the methyl ketones. Cultivation under microaerobic conditions improved titers and yields, and further engineering to knock out the native competitive pathways that become activated under microaerobic conditions led to significantly improved strains. The final strain, ΔadheΔldhaΔptaΔpoxB [pTrcHis2A-shmks2-mks1], produced up to 450 mg/L of methyl ketones at 17 mg of methyl ketones produced per gram of glucose consumed under optimized operating conditions in minimal media supplemented with glucose.en_US
dc.format.extent112 p.en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.callnoTHESIS CH.E. 2012 PARKen_US
dc.identifier.citationPark, John. "Engineering Escherichia coli for the production of polyketide-based platform chemicals." (2012) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/70384">https://hdl.handle.net/1911/70384</a>.en_US
dc.identifier.digitalParkJen_US
dc.identifier.urihttps://hdl.handle.net/1911/70384en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectApplied sciencesen_US
dc.subjectPure sciencesen_US
dc.subjectAlternative energyen_US
dc.subjectBiochemistryen_US
dc.subjectChemical engineeringen_US
dc.titleEngineering Escherichia coli for the production of polyketide-based platform chemicalsen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentChemical Engineeringen_US
thesis.degree.disciplineEngineeringen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Scienceen_US
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