Cloning and characterization of CMF, a density sensing factor in Dictyostelium discoideum
Abstract
Conditioned medium factor (CMF), is an 80 kD glycoprotein which is the ligand in a cell density sensing system used by developing Dictyostelium discoideum cells. CMF is slowly secreted by cells when they starve, and the extracellular level of CMF then becomes an indicator that is sensed to determine the density of starving cells.
CMF cDNA has been cloned and encodes a novel 62.6 kD protein. Bacterially synthesized recombinant CMF has as much CMF activity as native CMF, indicating that glycosylation is not required for the activity. The active site of the CMF protein lies within an 88 amino acid region near the N-terminus. Immunofluorescence with affinity-purified anti-CMF antibodies indicates that CMF is present in all vegetative and developing cells. In the vegetative cells, CMF is sequestered inside previously uncharacterized vesicles and begins to be slowly secreted upon starvation. CMF antisense transformants do not aggregate unless starved in the presence of exogenous CMF. To understand how CMF regulates the aggregation of cells, the effect of CMF on cAMP signal transduction was examined. The activation of Ca
Description
Advisor
Degree
Type
Keywords
Citation
Jain, Renu. "Cloning and characterization of CMF, a density sensing factor in Dictyostelium discoideum." (1994) Diss., Rice University. https://hdl.handle.net/1911/16742.