Key enzymes in parasite sterol metabolism

dc.contributor.advisorRudolph, Frederick B.en_US
dc.creatorJoubert, Bridget Maeen_US
dc.date.accessioned2009-06-04T08:44:27Zen_US
dc.date.available2009-06-04T08:44:27Zen_US
dc.date.issued2002en_US
dc.description.abstractThe lanosterol synthases from Trypanosoma cruzi, Trypanosoma brucei and Pneumocystis carinii, the lanosterol 14-demethylases from Trypanosoma brucei and Trypanosoma cruzi, and the cytochrome P450 reductase from Trypanosoma brucei were cloned and characterized. The two trypanosome lanosterol synthases showed a novel difference in protein sequence identity from that of other lanosterol synthases, which could be exploited for the development of specific antitrypanosome inhibitors. Yeast strains for expressing lanosterol 14alpha-demethylases were also developed. The first strains developed by tetrad dissection were time-consuming to produce, therefore another expression system was developed. The new system involved transforming the existing yeast strains, BJY1[pTb14DM] or BJY5[pTb14DM], and selection on FOA medium. With the addition of the T. brucei P450 reductase characterized in this study, the trypanosome lanosterol 14alpha-demethylases were able to regenerate their catalytic activity more efficiently than the strains containing only the native yeast P450 reductase. The various yeast strains developed in this study should be useful for screening antiparasite drugs. The BJY1[pTb14DM] (without reductase) and BJY5[pTb14DM] (with reductase) strains would also be useful in creating expression strains for other 14DM genes. Hopefully, these will be cloned in the near future.en_US
dc.format.extent114 p.en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.callnoTHESIS BIOCHEM. 2002 JOUBERTen_US
dc.identifier.citationJoubert, Bridget Mae. "Key enzymes in parasite sterol metabolism." (2002) Diss., Rice University. <a href="https://hdl.handle.net/1911/18095">https://hdl.handle.net/1911/18095</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/18095en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectBiochemistryen_US
dc.titleKey enzymes in parasite sterol metabolismen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentBiochemistry and Cell Biologyen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelDoctoralen_US
thesis.degree.nameDoctor of Philosophyen_US
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