Investigation and Development of Methods to Produce Budding Yeast Transcriptomic Aging Data

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dc.contributor.advisorDang, Weiweien_US
dc.contributor.advisorDiehl, Michaelen_US
dc.creatorNikoloutsos, Nicolasen_US
dc.date.accessioned2025-05-29T19:32:28Zen_US
dc.date.available2025-05-29T19:32:28Zen_US
dc.date.created2025-05en_US
dc.date.issued2025-04-22en_US
dc.date.submittedMay 2025en_US
dc.date.updated2025-05-29T19:32:28Zen_US
dc.description.abstractGiven the resources and proper environment, an isogenic S. cerevisiae population has the potential to propagate indefinitely. However, the individual yeast cells within a colony are not immortal and rejuvenation through budding is required for the continual production of youthful progeny. The rejuvenation process in yeast budding has been shown to be a victim of aging too, resulting in several known disparities between early and late daughter cells from the same mother cell. The transcriptomic signatures of sets of individual yeast daughter, or progeny, cells from throughout the entire lifespans of yeast mother, or progenitor, cells would reveal the changes that result from the age-induced breakdown of yeast budding rejuvenation and provide a meaningful measurement of biological aging. Using such data, it would be possible to construct a yeast single-cell transcriptomic aging landscape defining cellular biological age in an unbiased manner for yeast daughter cells. Further coupling the daughter series transcriptomes with those of yeast mother-daughter pairs would allow for the extension of the daughter cell aging landscape to a general yeast aging landscape which would detail the biological age of a yeast cell given its transcriptome. Currently, methods to capture single-cell yeast lineage data are lacking and need to be developed. As my thesis project, I developed and analyzed methods for plate-based single-cell and a bulk single-cell sequencing methods for budding yeast to be used for the establishment of a transcriptomic definition of cellular biological age at single-cell resolution which will further improve the utility of yeast as a eukaryotic cellular aging model organism.en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.urihttps://hdl.handle.net/1911/118416en_US
dc.language.isoenen_US
dc.subjectAgingen_US
dc.subjectyeasten_US
dc.subjecten_US
dc.titleInvestigation and Development of Methods to Produce Budding Yeast Transcriptomic Aging Dataen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentBioengineeringen_US
thesis.degree.disciplineBioengineeringen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelDoctoralen_US
thesis.degree.nameDoctor of Philosophyen_US
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