Biofilm-Mediated Regulation of Siderophore Production in Pseudomonas aeruginosa

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dc.contributor.advisorKirienko, Natalia Ven_US
dc.contributor.advisorBennett, George Nen_US
dc.creatorKang, Donghoonen_US
dc.date.accessioned2019-07-17T15:56:38Zen_US
dc.date.available2019-07-17T15:56:38Zen_US
dc.date.created2019-08en_US
dc.date.issued2019-06-04en_US
dc.date.submittedAugust 2019en_US
dc.date.updated2019-07-17T15:56:39Zen_US
dc.description.abstractPseudomonas aeruginosa is a gram-negative, multidrug-resistant, nosocomial pathogen that threatens the lives of immunocompromised patients. A key virulence factor in this pathogen is the siderophore pyoverdine. Due to its extremely high affinity for ferric iron, pyoverdine gives the pathogen a significant advantage over the host in their competition for iron. In addition, pyoverdine can regulate the production of multiple bacterial virulence factors and perturb host mitochondrial homeostasis. To elucidate the regulation of pyoverdine production, we developed a high-throughput genetic screen to identify genes necessary for its biosynthesis. Through this screen, we demonstrated that biofilm formation is necessary for pyoverdine production. Consistent with this result, upstream regulators of biofilm, notably intracellular c-di-GMP, regulated pyoverdine production in a biofilm-dependent manner. Furthermore, inhibiting biofilm formation using 2-amino-5,6-dimethylbenzimidazole was sufficient to attenuate pyoverdine production, suggesting that biofilm inhibitors may have multiple therapeutic benefits in addition to disrupting the pathogen’s main mechanism of antimicrobial resistance.en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationKang, Donghoon. "Biofilm-Mediated Regulation of Siderophore Production in Pseudomonas aeruginosa." (2019) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/106150">https://hdl.handle.net/1911/106150</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/106150en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectBiofilmen_US
dc.subjectPyoverdineen_US
dc.subjectPseudomonas aeruginosaen_US
dc.subjectAntivirulenceen_US
dc.subjectIron Acquisitionen_US
dc.subjectSiderophoresen_US
dc.subjectHigh-Throughput Screenen_US
dc.subjectc-di-GMPen_US
dc.subjectVirulenceen_US
dc.subjectCell Aggregationen_US
dc.titleBiofilm-Mediated Regulation of Siderophore Production in Pseudomonas aeruginosaen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentBiochemistry and Cell Biologyen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.majorMicrobiologyen_US
thesis.degree.nameMaster of Artsen_US
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