Quantifying DNA Melting Transitions Using Single-Molecule Force Spectroscopy

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dc.contributor.authorCalderon, Christopher P.
dc.contributor.authorChen, Wei-Hung
dc.contributor.authorLin, Kuan-Jiuh
dc.contributor.authorHarris, Nolan C.
dc.contributor.authorKiang, Ching-Hwa
dc.date.accessioned2018-06-19T17:13:02Z
dc.date.available2018-06-19T17:13:02Z
dc.date.issued2008-09
dc.date.noteSeptember 2008
dc.description.abstractWe stretched a DNA molecule using atomic force microscope and quantified the mechanical properties associated withﾠBandﾠSﾠforms of double-stranded DNA (dsDNA), molten DNA, and single-stranded DNA (ssDNA). We also fit overdamped diffusion models to the AFM time series and used these models to extract additional kinetic information about the system. Our analysis provides additional evidence supporting the view that S-DNA is a stable intermediate encountered during dsDNA melting by mechanical force. In addition, we demonstrated that the estimated diffusion models can detect dynamical signatures of conformational degrees of freedom not directly observed in experiments.
dc.format.extent12 pp
dc.identifier.citationCalderon, Christopher P., Chen, Wei-Hung, Lin, Kuan-Jiuh, et al.. "Quantifying DNA Melting Transitions Using Single-Molecule Force Spectroscopy." (2008) <a href="https://hdl.handle.net/1911/102098">https://hdl.handle.net/1911/102098</a>.
dc.identifier.urihttps://hdl.handle.net/1911/102098
dc.language.isoeng
dc.titleQuantifying DNA Melting Transitions Using Single-Molecule Force Spectroscopy
dc.typeTechnical report
dc.type.dcmiText
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