Source zone bioremediation of chlorobenzene DNAPLs: Performance assessment using real time quantitative polymerase chain reaction

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dc.contributor.advisorAlvarez, Pedro J.
dc.creatorDominguez-Faus, Rosa
dc.date.accessioned2009-06-03T21:07:43Z
dc.date.available2009-06-03T21:07:43Z
dc.date.issued2007
dc.description.abstractAquifer flow-through columns were operated for 12 weeks to evaluate the benefits of oxygen addition on the biodegradation of a Chlroboenzene (CB) DNAPL source zone. Quantitative PCR was used to measure total bacteria (16S rDNA) and several aromatic oxygenase genes. CB removal was faster in the oxygen-amended columns compared to a control column, and qPCR showed that whereas the biphenyl and toluene dioxygenase biomarkers were most abundant, increases in the biomarker concentration for the phenol hydroxylase gene reflected best the higher CB removal due to aerobic biostimulation. DGGE analyses of the soil amended with oxygen revealed the dominant presence of Rhodococcus erythropolis (89% sequence similarity), which belongs to a genus known for its ability to degrade many priority pollutants, including CBs.
dc.format.extent42 p.en_US
dc.format.mimetypeapplication/pdf
dc.identifier.callnoTHESIS C.E. 2007 DOMINGUEZ-FAUS
dc.identifier.citationDominguez-Faus, Rosa. "Source zone bioremediation of chlorobenzene DNAPLs: Performance assessment using real time quantitative polymerase chain reaction." (2007) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/20501">https://hdl.handle.net/1911/20501</a>.
dc.identifier.urihttps://hdl.handle.net/1911/20501
dc.language.isoeng
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.
dc.subjectEnvironmental science
dc.subjectEnvironmental engineering
dc.titleSource zone bioremediation of chlorobenzene DNAPLs: Performance assessment using real time quantitative polymerase chain reaction
dc.typeThesis
dc.type.materialText
thesis.degree.departmentChemical and Biomolecular Engineering
thesis.degree.disciplineEngineering
thesis.degree.grantorRice University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science
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