Hybrid Protein-Polymer Materials and their Applications
| dc.contributor.advisor | Ball, Zachary T | en_US |
| dc.creator | Larkin, James Owen | en_US |
| dc.date.accessioned | 2025-01-16T20:01:48Z | en_US |
| dc.date.available | 2025-01-16T20:01:48Z | en_US |
| dc.date.created | 2024-12 | en_US |
| dc.date.issued | 2024-09-13 | en_US |
| dc.date.submitted | December 2024 | en_US |
| dc.date.updated | 2025-01-16T20:01:48Z | en_US |
| dc.description.abstract | The conjugation of biomacromolecules, such as proteins, to polymeric materials has many applications. These applications are as varied as the formation of protein−polymer conjugates used in therapeutic treatments to applications in sensors, biocatalysts, and tools for separation of biomolecules. The diverse range of hybrid materials available necessitates a diverse range of corresponding methodologies to support their construction. Among the key focuses of this thesis includes methodologies for the development of protein−polymer biomaterials and their wide-ranging applications. The first chapter is a review of methods of site-selective protein conjugation with polymers via naturally encoded sequences. This review covers a variety of methodologies for protein−polymer conjugation moving from non-specific methods to more sophisticated, site-selective methods. The second chapter will review the structure and applications of protein-biomaterials, such as those conjugated to a nano-object or immobilized to a solid substrate. The second chapter will also cover the enhanced properties of novel materials at the interphase between nano, surface, and biological chemistry. The development of a boronic acid resin for the selective immobilization of canonically encoded (pyroglutamate-histidine-tagged) proteins is covered in the third chapter. The fourth chapter demonstrates a unique application of these protein−polymer biomaterials as a template in the synthesis of fluorescent copper nanoclusters. The fifth chapter will focus on efforts towards the controlled release of boronic acid-based therapeutics by tailoring boronate ester hydrolysis kinetics. Finally, the sixth chapter will showcase the antibacterial activity of capacitively coupled plasma from laser-induced graphene and the experiments elucidating the molecular mediator of bacterial cell death. | en_US |
| dc.format.mimetype | application/pdf | en_US |
| dc.identifier.uri | https://hdl.handle.net/1911/118182 | en_US |
| dc.language.iso | en | en_US |
| dc.subject | Fluorescence | en_US |
| dc.subject | Immobilization | en_US |
| dc.subject | Organic Polymers | en_US |
| dc.subject | Peptides And Proteins | en_US |
| dc.subject | Nanoclusters | en_US |
| dc.subject | Graphene | en_US |
| dc.subject | Cold Plasma | en_US |
| dc.subject | Ozone | en_US |
| dc.title | Hybrid Protein-Polymer Materials and their Applications | en_US |
| dc.type | Thesis | en_US |
| dc.type.material | Text | en_US |
| thesis.degree.department | Chemistry | en_US |
| thesis.degree.discipline | Chemistry | en_US |
| thesis.degree.grantor | Rice University | en_US |
| thesis.degree.level | Doctoral | en_US |
| thesis.degree.name | Doctor of Philosophy | en_US |
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