Gas chromatography and gateway sensors for on-line state estimation of complex fermentations (butanol/acetone fermentations)

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dc.contributor.advisorPapoutsakis, E. Terry
dc.contributor.committeeMemberRudolph, Frederick B.
dc.contributor.committeeMemberMcIntire, Larry V.
dc.creatorMcLaughlin, Joseph K.
dc.date.accessioned2018-12-18T21:16:20Z
dc.date.available2018-12-18T21:16:20Z
dc.date.issued1984
dc.description.abstractA bench-scale system for monitoring butanol/acetone fermentations was designed to demonstrate the use of on-line gas chromatography (GC) and the applicability of an "advanced" fermentation equation. Ultrafiltration was used to obtain sterilely a cell-free sample from a controlled fermentor for on-line GC analysis of ethanol, acetone, acetate, butanol, acetoin, and butyrate. The fermentor effluent gas was also analyzed by on-line GC for nitrogen, carbon monoxide, and carbon dioxide. Analytical data were automatically transmitted to a VAX 11/75 computer, for data archiving and fermentation equation calculations. The system was demonstrated in studies of pH and CO effects on the metabolism of Clostridium acetobutylicum. The on-line analytical systems provided excellent real time data. Also, three potentially useful gateway sensors were developed from the fermentation equation: glucose concentration, NADH2 from FdH2 and excess ATP. These demonstrated the usefulness of the fermentation equation for determination of intracellular and extracellular parameters in transient operation.
dc.format.digitalOriginreformatted digital
dc.format.extent202 pp
dc.identifier.callnoTHESIS CH.E. 1984 MCLAUGHLIN
dc.identifier.citationMcLaughlin, Joseph K.. "Gas chromatography and gateway sensors for on-line state estimation of complex fermentations (butanol/acetone fermentations)." (1984) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/104022">https://hdl.handle.net/1911/104022</a>.
dc.identifier.digitalRICE1648
dc.identifier.urihttps://hdl.handle.net/1911/104022
dc.language.isoeng
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.
dc.titleGas chromatography and gateway sensors for on-line state estimation of complex fermentations (butanol/acetone fermentations)
dc.typeThesis
dc.type.materialText
thesis.degree.departmentChemical Engineering
thesis.degree.disciplineEngineering
thesis.degree.grantorRice University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science
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