MSANTD3, a noval transcription factor, recruits PRC2 complex to regulate neuron differentiation in mouse P19 cells

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dc.contributor.advisorMa, Jianpengen_US
dc.contributor.committeeMemberDiehl, Michaelen_US
dc.contributor.committeeMemberTao, Yizhien_US
dc.creatorGou, Yufengen_US
dc.date.accessioned2016-01-14T22:02:34Zen_US
dc.date.available2016-01-14T22:02:34Zen_US
dc.date.created2014-12en_US
dc.date.issued2014-12-05en_US
dc.date.submittedDecember 2014en_US
dc.date.updated2016-01-14T22:02:34Zen_US
dc.description.abstractPolycomb repressive complex (PRC) 2 functions to repress thousands of target genes, and they are responsible for stem cell differentiation and carcinogenesis. However, how PRC2 are recruited to specific regions of their target genes remains elusive. In Drosophila, nine sequence-specific transcription factors including Zeste have been shown to act as PRC2 recruiters, but little is known about their homologues in mammalian cells, as a straightforward homology search failed to work in most cases. Aided by three-dimensional structure prediction and the use of the genomes of intermediate bridging species, we have identified a new protein, Myb/SANT-Like DNA-Binding Domain-Containing Protein 3 (MSANTD3), as the human homologue of Drosophila Zeste. Gel shift assays indicated that MSANTD3 binds to Zeste recognition sequence via its N-terminal DNA binding domain. MSANTD3 physically interacted with the components of PRC2 in GST-pull down assays and can be co-purified from native mouse P19 cells with active tri-methylation activity. Chip-seq and Chip-qPCR experiments showed the co-localization of MSANTD3 with PRC2 complex on neuron differentiation genes. Together, these findings revealed the critical function of MSANTD3 in recruiting PRC2 complexes to regulate neuron differentiation.en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationGou, Yufeng. "MSANTD3, a noval transcription factor, recruits PRC2 complex to regulate neuron differentiation in mouse P19 cells." (2014) Diss., Rice University. <a href="https://hdl.handle.net/1911/87825">https://hdl.handle.net/1911/87825</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/87825en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectMSANTD3en_US
dc.subjectPRC2en_US
dc.subjectneuron differentiationen_US
dc.titleMSANTD3, a noval transcription factor, recruits PRC2 complex to regulate neuron differentiation in mouse P19 cellsen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentApplied Physicsen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelDoctoralen_US
thesis.degree.majorApplied Physics/Bioengineeringen_US
thesis.degree.nameDoctor of Philosophyen_US
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