MSANTD3, a noval transcription factor, recruits PRC2 complex to regulate neuron differentiation in mouse P19 cells

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dc.contributor.advisorMa, Jianpeng
dc.contributor.committeeMemberDiehl, Michael
dc.contributor.committeeMemberTao, Yizhi
dc.creatorGou, Yufeng
dc.date.accessioned2016-01-14T22:02:34Z
dc.date.available2016-01-14T22:02:34Z
dc.date.created2014-12
dc.date.issued2014-12-05
dc.date.submittedDecember 2014
dc.date.updated2016-01-14T22:02:34Z
dc.description.abstractPolycomb repressive complex (PRC) 2 functions to repress thousands of target genes, and they are responsible for stem cell differentiation and carcinogenesis. However, how PRC2 are recruited to specific regions of their target genes remains elusive. In Drosophila, nine sequence-specific transcription factors including Zeste have been shown to act as PRC2 recruiters, but little is known about their homologues in mammalian cells, as a straightforward homology search failed to work in most cases. Aided by three-dimensional structure prediction and the use of the genomes of intermediate bridging species, we have identified a new protein, Myb/SANT-Like DNA-Binding Domain-Containing Protein 3 (MSANTD3), as the human homologue of Drosophila Zeste. Gel shift assays indicated that MSANTD3 binds to Zeste recognition sequence via its N-terminal DNA binding domain. MSANTD3 physically interacted with the components of PRC2 in GST-pull down assays and can be co-purified from native mouse P19 cells with active tri-methylation activity. Chip-seq and Chip-qPCR experiments showed the co-localization of MSANTD3 with PRC2 complex on neuron differentiation genes. Together, these findings revealed the critical function of MSANTD3 in recruiting PRC2 complexes to regulate neuron differentiation.
dc.format.mimetypeapplication/pdf
dc.identifier.citationGou, Yufeng. "MSANTD3, a noval transcription factor, recruits PRC2 complex to regulate neuron differentiation in mouse P19 cells." (2014) Diss., Rice University. <a href="https://hdl.handle.net/1911/87825">https://hdl.handle.net/1911/87825</a>.
dc.identifier.urihttps://hdl.handle.net/1911/87825
dc.language.isoeng
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.
dc.subjectMSANTD3
dc.subjectPRC2
dc.subjectneuron differentiation
dc.titleMSANTD3, a noval transcription factor, recruits PRC2 complex to regulate neuron differentiation in mouse P19 cells
dc.typeThesis
dc.type.materialText
thesis.degree.departmentApplied Physics
thesis.degree.disciplineNatural Sciences
thesis.degree.grantorRice University
thesis.degree.levelDoctoral
thesis.degree.majorApplied Physics/Bioengineering
thesis.degree.nameDoctor of Philosophy
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