Laser-based microfabrication for cell adhesion and migration

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dc.contributor.advisorWest, Jennifer L.en_US
dc.creatorMiller, Jordan S.en_US
dc.date.accessioned2009-06-03T19:53:16Zen_US
dc.date.available2009-06-03T19:53:16Zen_US
dc.date.issued2008en_US
dc.description.abstractMammalian cell adhesion and migration impact a multitude of cellular behaviors and tissue remodeling processes. Over the past several decades, investigators have methodically improved in vitro systems as mimics of the extracellular microenvironment to study these biologic phenomena. Experiments have progressed from early studies on bifunctional inorganic surfaces to those with purified adhesive proteins against an organic, non-adhesive background. Recently, subcellular geometric patterns of adhesive proteins have proven useful to restrict and direct focal contact formation, cell survival, lamellopodia extension, and the maturation of "supermature" focal contacts. The vast majority of recent studies have involved the construction of hydrophobic patches with adsorbed fibronectin as the adhesive constraint of choice. However, the extracellular matrix (ECM) in which cells operate is a complex and diverse environment where numerous signals interact with a cell simultaneously; signals that the cell must integrate and that directly impact these processes. Microfabrication methods to approximate the extracellular milieu have significant limitations in their potential to be extended to pattern multiple bioactive ligands with high precision. Current techniques require multi-step processes which lose feature fidelity at every pattern transfer step, while simultaneously increasing logistical complexity and the chance of technical missteps. We have developed a family of complementary techniques using the raster-scanning laser of a confocal microscope to address a number of current challenges in improving microfabrication. For our work with thin films of self-assembled organic monolayers, we systematically removed the multi-step processing requirements of conventional photolithographic microfabrication and characterized and verified the technical advantages of our new patterning techniques. For 3D work, we developed and demonstrated micron-scale biochemical and mechanical modifications of pre-formed photoactive hydrogels. These new microfabrication methods were then applied to direct and modulate cytoskeletal organization and cell migration. This work is broadly applicable to investigating fundamental cell-receptor, cell-substrate, and cell-cell interactions at the subcellular level, and may allow unprecedented and transformative studies of ligand arrangement and concentration effects on cell biology through the construction of multifaceted micropatterns in both two- and three-dimensions.en_US
dc.format.extent152 p.en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.callnoTHESIS BIOENG. 2008 MILLERen_US
dc.identifier.citationMiller, Jordan S.. "Laser-based microfabrication for cell adhesion and migration." (2008) Diss., Rice University. <a href="https://hdl.handle.net/1911/22276">https://hdl.handle.net/1911/22276</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/22276en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectCell biologyen_US
dc.subjectBiomedical engineeringen_US
dc.subjectEngineeringen_US
dc.subjectMaterials scienceen_US
dc.titleLaser-based microfabrication for cell adhesion and migrationen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentBioengineeringen_US
thesis.degree.disciplineEngineeringen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelDoctoralen_US
thesis.degree.nameDoctor of Philosophyen_US
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