Exploration of DNA binding selectivity, transcriptional regulation, and evolutionary relationships of the Hox protein Ultrabithorax

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dc.contributor.advisorMatthews, Kathleen S.
dc.creatorLiu, Ying
dc.date.accessioned2018-12-03T18:32:14Z
dc.date.available2018-12-03T18:32:14Z
dc.date.issued2007
dc.description.abstractHox transcription factors direct differentiation in all tissue layers and many organ systems and therefore have highly specific tissue-dependent functions. The DNA-binding homeodomain of Hox proteins can bind a wide array of nucleotide sequences with similar high affinities, suggesting regions outside the homeodomain must impact DNA interaction. Utilizing a series of deletion mutants, we identified three binding affinity modulation regions outside the homeodomain. DNA binding is inhibited ∼2-fold by the YPWM motif and microexon regions (I1) and ∼40-fold by the large disordered I2 region. High affinity binding is partially restored by the N-terminal 174 residues (R region) in a length-dependent manner. Both I2 and R regions partially overlap the Ubx transcription activation domain, allowing communication between these functional systems. Evolutionary variations in the amino acid sequences of most of these regions potentially differentiate Ubx•DNA interactions.
dc.format.extent261 pp
dc.identifier.callnoTHESIS BIOCHEM. 2008 LIU
dc.identifier.citationLiu, Ying. "Exploration of DNA binding selectivity, transcriptional regulation, and evolutionary relationships of the Hox protein Ultrabithorax." (2007) Diss., Rice University. <a href="https://hdl.handle.net/1911/103652">https://hdl.handle.net/1911/103652</a>.
dc.identifier.digital304815410
dc.identifier.urihttps://hdl.handle.net/1911/103652
dc.language.isoeng
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.
dc.subjectBiophysics
dc.subjectBiological sciences
dc.subjectDNA binding Hox proteins
dc.subjectTranscriptional regulation
dc.subjectUltrabithorax
dc.titleExploration of DNA binding selectivity, transcriptional regulation, and evolutionary relationships of the Hox protein Ultrabithorax
dc.typeThesis
dc.type.materialText
thesis.degree.departmentBiochemistry and Cell Biology
thesis.degree.disciplineNatural Sciences
thesis.degree.grantorRice University
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy
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