Exploration of DNA binding selectivity, transcriptional regulation, and evolutionary relationships of the Hox protein Ultrabithorax

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dc.contributor.advisorMatthews, Kathleen S.en_US
dc.creatorLiu, Yingen_US
dc.date.accessioned2018-12-03T18:32:14Zen_US
dc.date.available2018-12-03T18:32:14Zen_US
dc.date.issued2007en_US
dc.description.abstractHox transcription factors direct differentiation in all tissue layers and many organ systems and therefore have highly specific tissue-dependent functions. The DNA-binding homeodomain of Hox proteins can bind a wide array of nucleotide sequences with similar high affinities, suggesting regions outside the homeodomain must impact DNA interaction. Utilizing a series of deletion mutants, we identified three binding affinity modulation regions outside the homeodomain. DNA binding is inhibited ∼2-fold by the YPWM motif and microexon regions (I1) and ∼40-fold by the large disordered I2 region. High affinity binding is partially restored by the N-terminal 174 residues (R region) in a length-dependent manner. Both I2 and R regions partially overlap the Ubx transcription activation domain, allowing communication between these functional systems. Evolutionary variations in the amino acid sequences of most of these regions potentially differentiate Ubx•DNA interactions.en_US
dc.format.extent261 ppen_US
dc.identifier.callnoTHESIS BIOCHEM. 2008 LIUen_US
dc.identifier.citationLiu, Ying. "Exploration of DNA binding selectivity, transcriptional regulation, and evolutionary relationships of the Hox protein Ultrabithorax." (2007) Diss., Rice University. <a href="https://hdl.handle.net/1911/103652">https://hdl.handle.net/1911/103652</a>.en_US
dc.identifier.digital304815410en_US
dc.identifier.urihttps://hdl.handle.net/1911/103652en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectBiophysicsen_US
dc.subjectBiological sciencesen_US
dc.subjectDNA binding Hox proteinsen_US
dc.subjectTranscriptional regulationen_US
dc.subjectUltrabithoraxen_US
dc.titleExploration of DNA binding selectivity, transcriptional regulation, and evolutionary relationships of the Hox protein Ultrabithoraxen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentBiochemistry and Cell Biologyen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelDoctoralen_US
thesis.degree.nameDoctor of Philosophyen_US
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