Flavodoxin protein electron carriers: bioinformatic analysis and interactions with sulfite reductases

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dc.contributor.advisorSilberg, Jonathan J.en_US
dc.contributor.committeeMemberBennett, George N.en_US
dc.contributor.committeeMemberAjo-Franklin, Caroline M.en_US
dc.creatorGuseva, Annaen_US
dc.date.accessioned2020-08-14T18:31:36Zen_US
dc.date.available2020-08-14T18:31:36Zen_US
dc.date.created2020-08en_US
dc.date.issued2020-08-14en_US
dc.date.submittedAugust 2020en_US
dc.date.updated2020-08-14T18:31:36Zen_US
dc.description.abstractFlavodoxins (Flds) are oxidoreductases that distribute electrons to different metabolic pathways through interactions with an array of partner proteins. The aim of my thesis is to understand Fld evolution, establish whether Flds are encoded within the same genomes as Fd-dependent sulfite reductases (SIRs), and demonstrate that a cellular assay can monitor Fld electron transfer (ET) to SIRs. Using bioinformatics, I identify numerous microbes whose genomes encode both Fld and SIR genes. Additionally, I show that Flds can support ET to SIR using a synthetic pathway where protein-mediated ET is monitored using the growth of an Escherichia coli auxotroph that depends upon Fld transferring electrons from a Fd:NADP+ reductase to SIR. My results represent the first evidence that Flds support ET to assimilatory SIRs. Additionally, they show how a synthetic ET pathway in cells can be leveraged to rapidly compare the ET efficiencies of different Flds.en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationGuseva, Anna. "Flavodoxin protein electron carriers: bioinformatic analysis and interactions with sulfite reductases." (2020) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/109196">https://hdl.handle.net/1911/109196</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/109196en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectferredoxinen_US
dc.subjectflavodoxinen_US
dc.subjectsulfite reductaseen_US
dc.subjectsynthetic biologyen_US
dc.subjectbioinformaticsen_US
dc.titleFlavodoxin protein electron carriers: bioinformatic analysis and interactions with sulfite reductasesen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentBiochemistry and Cell Biologyen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Scienceen_US
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