Biosurfactant production by gasoline-degrading bacteria from a contaminated aquifer

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dc.contributor.advisorWard, C. H.
dc.creatorAllen, Phillip Glenn
dc.date.accessioned2009-06-04T00:03:24Z
dc.date.available2009-06-04T00:03:24Z
dc.date.issued1992
dc.description.abstractBiosurfactants produced by subsurface bacteria may potentially enhance in situ bioremediation. A study was performed to characterize the production and enhancement of production of biosurfactants by 37 gasoline-degrading bacteria isolated from a gasoline-contaminated aquifer. Surface tension and emulsification capacity of the bacterial cultures growing on unleaded gasoline vapors were determined and mean surface tension and emulsification capacity values calculated. When liquid gasoline was added to the cultures, the mean surface tension reduction of the sample population was nearly doubled, compared to the cultures using only gasoline vapors. Many of the bacterial isolates were able to grow without significant surface tension reductions or emulsification. For two select isolates, optimum ammonium sulfate concentrations for growth and biosurfactant production were determined. Addition of yeast extract enhanced biosurfactant production by the two isolates. Both isolates were shown to initiate production of biosurfactants during the stationary phase of cell growth.
dc.format.extent105 p.en_US
dc.format.mimetypeapplication/pdf
dc.identifier.callnoThesis Env. Sci. 1992 Allen
dc.identifier.citationAllen, Phillip Glenn. "Biosurfactant production by gasoline-degrading bacteria from a contaminated aquifer." (1992) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/13626">https://hdl.handle.net/1911/13626</a>.
dc.identifier.urihttps://hdl.handle.net/1911/13626
dc.language.isoeng
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.
dc.subjectEnvironmental science
dc.subjectMicrobiology
dc.subjectBiology
dc.titleBiosurfactant production by gasoline-degrading bacteria from a contaminated aquifer
dc.typeThesis
dc.type.materialText
thesis.degree.departmentEnvironmental Science
thesis.degree.disciplineEngineering
thesis.degree.grantorRice University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science
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