Mechanisms of cheating behavior in the social amoeba Dictyostelium discoideum

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dc.contributor.advisorStrassmann, Joan E.en_US
dc.creatorSantorelli, Lorenzo Andreaen_US
dc.date.accessioned2009-06-03T21:11:24Zen_US
dc.date.available2009-06-03T21:11:24Zen_US
dc.date.issued2007en_US
dc.description.abstractDictyostelium discoideum is a eukaryotic micro-organism with a unique life cycle. The amoebae live as haploid, free-living cells in the soil feeding on bacteria and dividing asexually. Under starvation conditions, the cells aggregate and undergo a process of differentiation into spores and stalk cells. We speculate that the stalk cells are sacrificed to help raise the spores above the substrate and to improve their dispersal and survival. In the case of a mix between two or more genetically different clones, a conflict may arise over which cells become spores and survive and which become stalk and die. One that differentiates more spores than its fair share in chimera is called a "cheater" and the other a "loser". Dictyostelium discoideum is a useful organism for studying the complexity of social behavior in microorganisms. Molecular tools have been developed allowing the study of genetic mechanisms that underlie this social behavior. To investigate the molecular basis of cooperation, several pools of knock-out mutants were generated using the REMI (R&barbelow;estriction E&barbelow;nzyme M&barbelow;ediated I&barbelow;ntegration) technique. To simulate evolutionary selection for cheaters, the different mutants were subjected to rounds of spore germination, growth and development in a mixed population. Only the spores were taken to the next generation. Real Time PCR confirmed that cheaters became over-represented in the evolving population because they contribute spores with a higher efficiency than the other mutants. Mutants expressing a normal phenotype were picked and isolated after 10 and 20 cycles of selection and mixed in pairwise experiments with the parental wild type. At least 35 mutants have been tested and 29 were cheaters. Analysis of the isolated genes suggested that several genetic pathways are involved in regulating or modulating the complex cooperation process in Dictyostelium discoideum. Finally we characterized one cheater mutant, called chtB, which shows apparently normal phenotype when plated clonally. The mutant is lacking in the expression of the gene chtB. In chimeras, this causes the reduction of the expression of the prespore marker cotB in the wild type strain, enabling the cheater to differentiate more spores than the parental strain.en_US
dc.format.extent112 p.en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.callnoTHESIS ECOL. 2007 SANTORELLIen_US
dc.identifier.citationSantorelli, Lorenzo Andrea. "Mechanisms of cheating behavior in the social amoeba Dictyostelium discoideum." (2007) Diss., Rice University. <a href="https://hdl.handle.net/1911/20645">https://hdl.handle.net/1911/20645</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/20645en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectGeneticsen_US
dc.subjectMicrobiologyen_US
dc.titleMechanisms of cheating behavior in the social amoeba Dictyostelium discoideumen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentEcology and Evolutionary Biologyen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelDoctoralen_US
thesis.degree.nameDoctor of Philosophyen_US
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