Single-Frame 3D Fluorescence Microscopy with Ultra-Miniature Lensless FlatScope

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dc.contributor.advisorRobinson, Jacob T
dc.contributor.committeeMemberBaraniuk, Richard G
dc.contributor.committeeMemberLandes, Christy
dc.creatorAdams, Jesse K
dc.date.accessioned2019-05-16T20:14:04Z
dc.date.available2019-05-16T20:14:04Z
dc.date.created2017-08
dc.date.issued2017-06-01
dc.date.submittedAugust 2017
dc.date.updated2019-05-16T20:14:04Z
dc.description.abstractModern biology increasingly relies on fluorescence microscopy, which is driving demand for smaller, lighter, and cheaper microscopes. However, traditional microscope architectures suffer from a fundamental tradeoff: as lenses become smaller, they must either collect less light or image a smaller field of view. To break this fundamental tradeoff between device size and performance, we present a new concept for 3D fluorescence imaging that replaces lenses with an optimized amplitude mask placed a few hundred microns above the sensor and an efficient algorithm that can convert a single frame of captured sensor data into high-resolution 3D images. The result is FlatScope: a lensless microscope that is scarcely larger than an image sensor (roughly 0.2 grams in weight and less than 1 mm thick) and yet able to produce micron-resolution, high-frame-rate, 3D fluorescence movies covering a total volume of several cubic millimeters. The ability of FlatScope to reconstruct full 3D images from a single frame of captured sensor data allows us to image 3D volumes roughly 40,000 times faster than a laser scanning confocal microscope. We envision that this new flat fluorescence microscopy paradigm will lead to implantable endoscopes that minimize tissue damage, arrays of imagers that cover large areas, and bendable, flexible microscopes that conform to complex topographies.
dc.format.mimetypeapplication/pdf
dc.identifier.citationAdams, Jesse K. "Single-Frame 3D Fluorescence Microscopy with Ultra-Miniature Lensless FlatScope." (2017) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/105488">https://hdl.handle.net/1911/105488</a>.
dc.identifier.urihttps://hdl.handle.net/1911/105488
dc.language.isoeng
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.
dc.subjectLens-free
dc.subjectWide-field
dc.subjectFluorescent
dc.subjectComputational Imaging
dc.subjectLenses
dc.subjectAlgorithms
dc.subjectUltra thin
dc.subjectVolumetric
dc.subjectFlat
dc.subject
dc.titleSingle-Frame 3D Fluorescence Microscopy with Ultra-Miniature Lensless FlatScope
dc.typeThesis
dc.type.dcmiMovingImage
dc.type.materialText
thesis.degree.departmentApplied Physics
thesis.degree.disciplineNatural Sciences
thesis.degree.grantorRice University
thesis.degree.levelMasters
thesis.degree.majorApplied Physics/Electrical Eng
thesis.degree.nameMaster of Science
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