Effects of mechanical loading on osteoblast function using a three dimensional celijpolymer model

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dc.contributor.advisorMcIntire, Larry V.en_US
dc.contributor.committeeMemberMikos, Antonios G.en_US
dc.contributor.committeeMemberRudolph, Frederick B.en_US
dc.contributor.committeeMemberGustin, Michael C.en_US
dc.contributor.committeeMemberFarach-Carson, Cindyen_US
dc.creatorJen, Anna Hsiao-Chiehen_US
dc.date.accessioned2014-08-08T22:00:27Zen_US
dc.date.available2014-08-08T22:00:27Zen_US
dc.date.issued1998en_US
dc.description.abstractMechanisms which trigger bone modeling/remodeling in response to changes in the mechanical environment are still unclear. In a three part study, effects of loading on osteoblast function were investigated using a three dimensional (3-D) ceWpolymer model. The 3-D model has advantages of cell culture while maintaining the natural matrix architecture of bone. Such celVpolymer constructs have been shown to form bone in vitro. Osteoblasts in 3-D ceWpolymer constructs were cyclically loaded (5% ). After five days, compressed constructs decreased in alkaline phosphatase activity, a marker of osteoblast maturation. After three weeks, loaded constructs showed lower alkaline phosphatase activity but higher RNA level of L-type calcium channels, involved in calcium signaling cascades. No difference was detected after twelve weeks. Results suggest osteoblasts sensed loading and altered functional activities in response. Use of the 3-D model to study other osteoblast functions under mechanical loading may increase understanding of regulated functional adaptation by bone.en_US
dc.format.extent108 ppen_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationJen, Anna Hsiao-Chieh. "Effects of mechanical loading on osteoblast function using a three dimensional celijpolymer model." (1998) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/76500">https://hdl.handle.net/1911/76500</a>.en_US
dc.identifier.digitalJenAen_US
dc.identifier.urihttps://hdl.handle.net/1911/76500en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectChemical engineeringen_US
dc.titleEffects of mechanical loading on osteoblast function using a three dimensional celijpolymer modelen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentChemical and Biomolecular Engineeringen_US
thesis.degree.disciplineEngineeringen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Scienceen_US
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