An optimized procedure to allow 3D optical imaging of biomarkers associated with neoplasia in the oral mucosa

dc.contributor.advisorRichards-Kortum, Rebeccaen_US
dc.creatorTran, Emilyen_US
dc.date.accessioned2017-07-31T14:39:32Zen_US
dc.date.available2017-12-01T06:01:04Zen_US
dc.date.created2016-12en_US
dc.date.issued2016-11-22en_US
dc.date.submittedDecember 2016en_US
dc.date.updated2017-07-31T14:39:32Zen_US
dc.description.abstractImplementation of an optical clearing technique, called the Passive Clarity Technique (PACT), in mouse tongues enables three-dimensional (3D) fluorescence imaging of intact oral epithelial tissue. Using 3D imaging to improve our understanding of the spatial and temporal patterns of biomarker expression and phenotypic features in oral pre-cancer can improve accuracy of early diagnostic tools. Current methods for 3D fluorescence imaging require thin sectioning and accurate image reconstruction or are limited in imaging depth by tissue scattering. Using a polymer-tissue hydrogel technique previously developed for brain tissue, light scattering molecules were removed from mouse tongues to improve tissue transparency and antibody permeability for imaging and immunolabeling. In this study, PACT-Cleared tongues resulted in resolution of tissue features up to 500 μm in depth, while preserving tissue architecture and molecular information in normal and precancerous tissue. These findings can facilitate further exploration of biomarkers of oral cancer progression in a mouse model.en_US
dc.embargo.terms2017-12-01en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationTran, Emily. "An optimized procedure to allow 3D optical imaging of biomarkers associated with neoplasia in the oral mucosa." (2016) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/95532">https://hdl.handle.net/1911/95532</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/95532en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectConfocal Microscopyen_US
dc.subjectOptical Clearingen_US
dc.subjectOral Canceren_US
dc.titleAn optimized procedure to allow 3D optical imaging of biomarkers associated with neoplasia in the oral mucosaen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentBioengineeringen_US
thesis.degree.disciplineEngineeringen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Scienceen_US
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