The Induction of Infectivity in Human Astrovirus in Response to Capsid Proteolysis

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dc.contributor.advisorTao, Yizhi Janeen_US
dc.contributor.committeeMemberMcNew, James A.en_US
dc.contributor.committeeMemberStewart, Charles R.en_US
dc.creatorHarper, Justinen_US
dc.date.accessioned2014-09-11T16:28:21Zen_US
dc.date.available2014-09-11T16:28:21Zen_US
dc.date.created2013-12en_US
dc.date.issued2013-06-28en_US
dc.date.submittedDecember 2013en_US
dc.date.updated2014-09-11T16:28:21Zen_US
dc.description.abstractAstrovirus is a non-enveloped, T=3, positive-sense RNA virus that presents with self-limiting gastroenteritis; however, it has been additionally associated with serious presentations such as nephritis, hepatitis, and encephalitis, which is compounded by its propensity to engage in cross-species penetrations. Astrovirus undergoes a complex capsid maturation process mediated by host proteases in which an inert, immature capsid composed of VP90 is sequentially cleaved to yield a highly infectious particle composed of VP34 and VP27/VP25, which form the capsid shell and spikes, respectively. By overexpressing a VP9070-418 truncate in insect cells, we have demonstrated that the shell domain alone cannot support particle assembly, implying a crucial role for the dimeric contacts within the spike. Various monomeric, shell domain truncates (i.e. VP9071-252, VP9071-283, VP9071-313, and VP9071-415) have been successfully expressed and purified, but none yielded useful crystals, suggesting the structural context of the capsid lattice may be needed to stabilize their conformational flexibility.en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationHarper, Justin. "The Induction of Infectivity in Human Astrovirus in Response to Capsid Proteolysis." (2013) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/77163">https://hdl.handle.net/1911/77163</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/77163en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectX-ray crystallographyen_US
dc.subjectVirologyen_US
dc.subjectAstrovirusen_US
dc.subjectInfectivityen_US
dc.subjectStructureen_US
dc.subjectAssemblyen_US
dc.subjectBiophysicsen_US
dc.subjectBiochemistryen_US
dc.subjectHAstVen_US
dc.subjectCapsiden_US
dc.subjectRNA virusen_US
dc.titleThe Induction of Infectivity in Human Astrovirus in Response to Capsid Proteolysisen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentBiochemistry and Cell Biologyen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Artsen_US
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