Molecular Imaging of Mucin-Expressing Colon tumors Using Targeted Hyperpolarized Silicon Nanoparticles

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dc.contributor.advisorConstantinou Papadopoulos, Pamelaen_US
dc.contributor.advisorCarson, Danielen_US
dc.creatorLiu, Julie Xinlien_US
dc.date.accessioned2017-08-01T15:39:10Zen_US
dc.date.available2017-08-01T15:39:10Zen_US
dc.date.created2016-12en_US
dc.date.issued2016-11-22en_US
dc.date.submittedDecember 2016en_US
dc.date.updated2017-08-01T15:39:10Zen_US
dc.description.abstractColon cancer remains a leading cause of death due to limitations in clinical detection and prevention. Accordingly, an improved method with higher degree of detection sensitivity is due, where tumor surface markers can be targeted, and non-invasively imaged. MUC1 is a transmembrane mucin that is normally expressed only the apical aspect of colonic epithelia. MUC1 is a heavily glycosylated, large molecular weight protein that extends 200 to 500 nm above the cell surface. Accompanying cellular transformation and tumor development, MUC1 often becomes aberrantly expressed, including loss of polarized expression and altered glycosylation. With MUC1 as target, MUC1 antibody-functionalized silicon nanoparticles (SiNP may be used as uniquely hyperpolarizable imaging agents for the ultimate purpose of in vivo clinical applications for early colon tumor detection. This study aims to develop MUC1-targeting SiNPs that can undergo the hyperpolarization process to be utilized as sensitive image contrast agents to detect MUC1-expressing tumors. With cancer-associated transmembrane mucins found in most GI tract cancers, this silicon nanoparticle-based tumor diagnostic approach offers the potential to be expanded to detect tumors of other GI tissues and organs and establishes the basis for using other surface markers as detection targets.en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationLiu, Julie Xinli. "Molecular Imaging of Mucin-Expressing Colon tumors Using Targeted Hyperpolarized Silicon Nanoparticles." (2016) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/95979">https://hdl.handle.net/1911/95979</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/95979en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectAPDMES (3-aminopropyl)-dimethyl-ethoxysilaneen_US
dc.subjectAPTES (3-Aminopropyl)triethoxysilaneen_US
dc.subjectBME β-Mercaptoethanolen_US
dc.subjectBSA bovine serum albuminen_US
dc.subjectCRC Colorectal canceren_US
dc.subjectDAPI 4',6-diamidino-2-phenylindoleen_US
dc.subjectDMEM Dulbecco's Modified Eagle Mediumen_US
dc.subjectDMSO Dimethyl sulfoxideen_US
dc.subjectFBS Fetal bovine serumen_US
dc.subjectHBSS Hanks balanced salt solutionen_US
dc.subjectHEPES 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic aciden_US
dc.subjectMRI magnetic resonance imagingen_US
dc.subjectNEAA non-essential amino aciden_US
dc.subjectNHS N-hydrozysuccinimideen_US
dc.subjectPBS Phosphate-buffered salineen_US
dc.subjectPBST Phosphate-buffered saline with 0.1% (v/v) Tween-20en_US
dc.subjectPEG Polyethylene glycolen_US
dc.subjectRPMI Roswell Park Memorial Institute mediumen_US
dc.subjectSiP Silicon particleen_US
dc.subjectSiNP Silicon nanoparticleen_US
dc.subjectTCEP Tris(2-carboxyethyl1)phosphineen_US
dc.titleMolecular Imaging of Mucin-Expressing Colon tumors Using Targeted Hyperpolarized Silicon Nanoparticlesen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentBiochemistry and Cell Biologyen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Artsen_US
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