Molecular Imaging of Mucin-Expressing Colon tumors Using Targeted Hyperpolarized Silicon Nanoparticles

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dc.contributor.advisorConstantinou Papadopoulos, Pamela
dc.contributor.advisorCarson, Daniel
dc.creatorLiu, Julie Xinli
dc.date.accessioned2017-08-01T15:39:10Z
dc.date.available2017-08-01T15:39:10Z
dc.date.created2016-12
dc.date.issued2016-11-22
dc.date.submittedDecember 2016
dc.date.updated2017-08-01T15:39:10Z
dc.description.abstractColon cancer remains a leading cause of death due to limitations in clinical detection and prevention. Accordingly, an improved method with higher degree of detection sensitivity is due, where tumor surface markers can be targeted, and non-invasively imaged. MUC1 is a transmembrane mucin that is normally expressed only the apical aspect of colonic epithelia. MUC1 is a heavily glycosylated, large molecular weight protein that extends 200 to 500 nm above the cell surface. Accompanying cellular transformation and tumor development, MUC1 often becomes aberrantly expressed, including loss of polarized expression and altered glycosylation. With MUC1 as target, MUC1 antibody-functionalized silicon nanoparticles (SiNP may be used as uniquely hyperpolarizable imaging agents for the ultimate purpose of in vivo clinical applications for early colon tumor detection. This study aims to develop MUC1-targeting SiNPs that can undergo the hyperpolarization process to be utilized as sensitive image contrast agents to detect MUC1-expressing tumors. With cancer-associated transmembrane mucins found in most GI tract cancers, this silicon nanoparticle-based tumor diagnostic approach offers the potential to be expanded to detect tumors of other GI tissues and organs and establishes the basis for using other surface markers as detection targets.
dc.format.mimetypeapplication/pdf
dc.identifier.citationLiu, Julie Xinli. "Molecular Imaging of Mucin-Expressing Colon tumors Using Targeted Hyperpolarized Silicon Nanoparticles." (2016) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/95979">https://hdl.handle.net/1911/95979</a>.
dc.identifier.urihttps://hdl.handle.net/1911/95979
dc.language.isoeng
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.
dc.subjectAPDMES (3-aminopropyl)-dimethyl-ethoxysilane
dc.subjectAPTES (3-Aminopropyl)triethoxysilane
dc.subjectBME β-Mercaptoethanol
dc.subjectBSA bovine serum albumin
dc.subjectCRC Colorectal cancer
dc.subjectDAPI 4',6-diamidino-2-phenylindole
dc.subjectDMEM Dulbecco's Modified Eagle Medium
dc.subjectDMSO Dimethyl sulfoxide
dc.subjectFBS Fetal bovine serum
dc.subjectHBSS Hanks balanced salt solution
dc.subjectHEPES 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
dc.subjectMRI magnetic resonance imaging
dc.subjectNEAA non-essential amino acid
dc.subjectNHS N-hydrozysuccinimide
dc.subjectPBS Phosphate-buffered saline
dc.subjectPBST Phosphate-buffered saline with 0.1% (v/v) Tween-20
dc.subjectPEG Polyethylene glycol
dc.subjectRPMI Roswell Park Memorial Institute medium
dc.subjectSiP Silicon particle
dc.subjectSiNP Silicon nanoparticle
dc.subjectTCEP Tris(2-carboxyethyl1)phosphine
dc.titleMolecular Imaging of Mucin-Expressing Colon tumors Using Targeted Hyperpolarized Silicon Nanoparticles
dc.typeThesis
dc.type.materialText
thesis.degree.departmentBiochemistry and Cell Biology
thesis.degree.disciplineNatural Sciences
thesis.degree.grantorRice University
thesis.degree.levelMasters
thesis.degree.nameMaster of Arts
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