Simple ultraviolet microscope using off-the-shelf components for point-of-care diagnostics.

Simple item page
dc.contributor.orgBioengineeringen_US
dc.creatorWong, Cindyen_US
dc.date.accessioned2019-02-12T18:34:26Zen_US
dc.date.available2019-02-12T18:34:26Zen_US
dc.date.issued2019-02-12en_US
dc.description.abstractAt the primary care setting, where there are often no or minimal laboratories, examinations often consist of self-testing and rapid diagnostics. Because of this, medical devices must be simple, robust, and easy to operate. To address these concerns, an alternate fluorescence microscope design uses ultraviolet (UV) excitation, since fluorescent dyes that are excitable in the visible region are also excitable by UV. This may allow for the removal of typical excitation, emission, and dichroic filters as optical components absorb UV wavelengths and UV is not detected by silicon based detectors. Additionally, UV has a very low penetration into samples, which may allow for controlling the depth of excitation, and thus the imaging volume. Based on these ideas, we developed a simple fluorescence microscope built completely from off-the-shelf components that uses UV to image fluorescently stained samples. The simple opto-mechanical design of the system may allow it to be more compact and easy to use, as well as decrease the overall cost of the diagnostic device. For biological validation, we imaged whole blood stained with acridine orange and performed a two-part white blood cell differential count.en_US
dc.digitization.specificationsThe zip file contains 60 TIF files organized into subfolders per trail. There are 12 images per subfolder. Each image is 6.4 MB.en_US
dc.format.extent382 MBen_US
dc.format.mimetypeimage/tiffen_US
dc.identifier.citationWong, Cindy (2019): Simple ultraviolet microscope using off-the-shelf components for point-of-care diagnostics.. [Dataset]. Rice University. <a href="https://hdl.handle.net/1911/105155">https://hdl.handle.net/1911/105155</a>.en_US
dc.identifier.urihttps://hdl.handle.net/1911/105155en_US
dc.language.isoengen_US
dc.publisherRice Universityen_US
dc.subject.keywordUltravioleten_US
dc.subject.keywordfluorescence microscopyen_US
dc.subject.keywordwhite blood cellen_US
dc.titleSimple ultraviolet microscope using off-the-shelf components for point-of-care diagnostics.en_US
dc.title.subtitleDataseten_US
dc.type.dcmiDataseten_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
UVFluorescence_WBCDataSet.zip
Size:
237.43 MB
Format:
application/zip, application/x-compressed-zip
Description:
Download
Collections
Rice Research Data
Rice Graduate Student Collection