Development of a 3D Tissue Engineered Bone Tumor Model

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dc.contributor.advisorMikos, Antonios G.en_US
dc.contributor.committeeMemberLudwig, Joseph A.en_US
dc.contributor.committeeMemberKasper, Kurten_US
dc.contributor.committeeMemberJacot, Jeffrey G.en_US
dc.contributor.committeeMemberZygourakis, Kyriacosen_US
dc.creatorBurdett, Emilyen_US
dc.date.accessioned2013-09-16T14:53:03Zen_US
dc.date.accessioned2013-09-16T14:53:06Zen_US
dc.date.available2013-09-16T14:53:03Zen_US
dc.date.available2013-09-16T14:53:06Zen_US
dc.date.created2013-05en_US
dc.date.issued2013-09-16en_US
dc.date.submittedMay 2013en_US
dc.date.updated2013-09-16T14:53:06Zen_US
dc.description.abstract3D ex vivo tumor models are required which better replicate the microenvironment encountered by tumor cells in vivo. In this study, we applied bone tissue engineering culture techniques to develop an ex vivo 3D bone tumor model. Ewing sarcoma cells were cultured on poly(ε-caprolactone) (PCL) microfiber scaffolds, and cellular growth kinetics, morphology, and infiltration were assessed. Cell/scaffold constructs were then exposed to anticancer drugs for up to 16 days and drug response was compared to 2D controls. Ewing sarcoma cells were capable of attachment and proliferation on PCL scaffolds and dense scaffold infiltration up to 200 micrometers. Constructs could be maintained in culture for up to 32 days, and high density 3D cell growth conferred an increased resistance to anticancer drugs over 2D controls. This 3D tumor model shows potential for use in future studies of bone tumor biology, especially as it pertains to the development of new anticancer drugs.en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.citationBurdett, Emily. "Development of a 3D Tissue Engineered Bone Tumor Model." (2013) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/71931">https://hdl.handle.net/1911/71931</a>.en_US
dc.identifier.slug123456789/ETD-2013-05-573en_US
dc.identifier.urihttps://hdl.handle.net/1911/71931en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectTissue engineeringen_US
dc.subject3D cancer modelsen_US
dc.subjectEwing Sarcomaen_US
dc.subjectCancer drug discoveryen_US
dc.titleDevelopment of a 3D Tissue Engineered Bone Tumor Modelen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentBioengineeringen_US
thesis.degree.disciplineEngineeringen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Scienceen_US
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