A disposable bio-nano-chip usuing agarose beads for protein analysis

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dc.contributor.advisorMcDevitt, John T.en_US
dc.creatorDu, Nanen_US
dc.date.accessioned2013-03-08T00:33:28Zen_US
dc.date.available2013-03-08T00:33:28Zen_US
dc.date.issued2012en_US
dc.description.abstractThis thesis reports on the fabrication of a disposable bio-nano-chip (BNC), a microfluidic device composed of polydimethylsiloxane (PDMS) and thiolene-based optical epoxy which is both cost-effective and suitable for high performance immunoassays. A novel room temperature (RT) bonding technique was utilized so as to achieve irreversible covalent bonding between PDMS and thiolene-based epoxy layers, while at the same time being compatible with the insertion of agarose bead sensors, selectively arranged in an array of pyramidal microcavities replicated in the thiolene thin film layer. In the sealed device, the bead-supporting epoxy film is sandwiched between two PDMS layers comprising of fluidic injection and drain channels. The agarose bead sensors used in the device are sensitized with anti-C-reactive protein (CRP) antibody, and a fluorescent sandwich-type immunoassay was run to characterize the performance of this device. Computational fluid dynamics (CFD) was used based on the device specifications to model the bead penetration. Experimental data revealed analyte penetration of the immunocomplex to 100μm into the 280μm diameter agarose beads, which correlated well with the simulation. A dose response curve was obtained and the linear dynamic range of the assay was established over 1ng/mL to 50ng/mL with a limit of detection less than 1ng/mL.en_US
dc.format.extent36 p.en_US
dc.format.mimetypeapplication/pdfen_US
dc.identifier.callnoTHESIS PHYS. 2012 DUen_US
dc.identifier.citationDu, Nan. "A disposable bio-nano-chip usuing agarose beads for protein analysis." (2012) Master’s Thesis, Rice University. <a href="https://hdl.handle.net/1911/70232">https://hdl.handle.net/1911/70232</a>.en_US
dc.identifier.digitalDuNen_US
dc.identifier.urihttps://hdl.handle.net/1911/70232en_US
dc.language.isoengen_US
dc.rightsCopyright is held by the author, unless otherwise indicated. Permission to reuse, publish, or reproduce the work beyond the bounds of fair use or other exemptions to copyright law must be obtained from the copyright holder.en_US
dc.subjectApplied sciencesen_US
dc.subjectBiological sciencesen_US
dc.subjectBiomedical engineeringen_US
dc.subjectBiophysicsen_US
dc.titleA disposable bio-nano-chip usuing agarose beads for protein analysisen_US
dc.typeThesisen_US
dc.type.materialTexten_US
thesis.degree.departmentPhysicsen_US
thesis.degree.disciplineNatural Sciencesen_US
thesis.degree.grantorRice Universityen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Scienceen_US
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